摘要
目的:观察色胺酮对哮喘豚鼠血清及肺泡灌洗液中NO、NOS浓度的影响。方法:将初筛合格的48只豚鼠随机分成6组:空白组(A组)、慢性模型对照组(B组)、色胺酮高剂量组(C组)、色胺酮中剂量组(D组)、色胺酮低剂量组(E组)和阳性对照组(F组)。采用磷酸卵蛋白(OVA)腹腔注射致敏加雾化吸入激发的方法复制豚鼠哮喘模型,造模成功后给予色胺酮和地塞米松治疗。测定各组豚鼠肺部组织、血清及肺泡灌液(BALF)中NO和NOS浓度并进行组间比较。结果:与B组相比,C、D、E、F组血清中NO和NOS浓度显著降低,组间比较,差异存在统计学意义(P<0.05);各组肺组织中NOS表达呈阴性;C、D、E、F组BALF中NO浓度较B组降低,组间比较,差异存在统计学意义(P<0.05)。结论:色胺酮可降低哮喘豚鼠血清中NO和NOS水平,降低BALF中NO水平,可能对哮喘有一定的防治作用。
Objective： To investigate the effects of tryptanthrin on NO, NOS in BALF and serum of asthmatic guinea pig. Methods： Forty-eight pathogen-free male guinea pigs were randomly divided into 6 groups. These groups are marked as the normal control group （group A）, experimental control group（group B）, high dose group of tryptanthrin（group C）, middle dose group of tryptanthrin（group D）, low dose group of tryptanthrin （group） E and positive group （group F）. The asthmatic models were established by injecting ovalbumin （OVA） into abdomen and atomization, then treated with tryptanthrin or Dexamethasone. The levels of NO, NOS were detected in serum and BALF. Results： The level of NO, NOS in serum of therapeutic groups were significantly lower than that of group B, and the levels of NO in BALF of therapeutic groups were also significantly lower than that of group B. Conclusions： Tryptanthrin could decrease the levels of NO, NOS in serum and BALF of asthmatic guinea pigs, so it might have certain preventive and therapeutic effect on Asthma.
出处
《现代生物医学进展》
CAS
2009年第8期1531-1532,F0003,共3页
Progress in Modern Biomedicine
