摘要
目的:探讨水通道蛋白1(AQP1)和血管内皮生长因子(VEGF)在喉鳞状细胞癌(LSCC)组织中的表达及生物学意义,并探讨其相关性。方法:采用免疫组织化学方法对60例LSCC(喉癌组)、22例声带息肉组织(声带息肉组)及30例正常喉组织(对照组)中AQP1和VEGF的表达进行检测。结果:AQP1和VEGF在喉癌组的表达高于声带息肉组及对照组,3组之间差异有统计学意义;AQP1和VEGF的表达与LSCC的临床分期、病理分级以及淋巴结转移均相关,二者的表达随临床分期的增高而逐渐升高;组织分化程度越差,二者的表达越高;在有淋巴结转移的喉癌组织中二者的表达高于无淋巴结转移组;不同临床分型以及不同性别、年龄患者喉癌组中AQP1和VEGF的表达均差异无统计学意义;AQP1与VEGF在LSCC组织中的表达存在正相关。结论:LSCC组织中AQP1和VEGF蛋白的高表达及与LSCC临床病理参数的相关性,提示2个因子在LSCC发生、发展过程中起促进癌细胞生长、转移和血管生成的作用。
Objective：To study the expression and biological value of AQP1 and VEGF in laryngeal squamous cell carcinoma （LSCC） and their correlation. Method：The expressions of AQP1 and VEGF were detected with immunohistochemical SP method in 60 cases of LSCC, 22 cases of polyps of vocal cord and 30 cases of normal tissues. Result：The expression of AQP1 and VEGF in the laryngeal carcinoma group was significantly higher than that in polyps of vocal cord group and normal control. The expression of AQP1 and VEGF was related to the clinical staging, the differentiation degree and the lymph node metastasis. The higher was the tumor stage ,the stronger was AQP1 and VEGF expression;the lower was the tumor differentiation ,the stronger was AQP1 and VEGF expression. The expression of AQP1 and VEGF in the group with lymph node metastasis was higher than that in non metastasis ones;The sexuality and age did not affect the expressions of AQP1 and VEGF. The expressions was direct correlation between AQP1 and VEGF in the laryngeal carcinoma tissues. Conclusion：The high expressions of AQP1 and VEGF and the close relationship with the clinical pathological factors in the LSCC, implicated that AQP1 and VEGF played an important role in the laryngeal carcinoma cells growth,metastasis and angiogenesis of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2009年第9期407-410,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
