摘要
利用超滤膜分离装置对白术多糖进行超滤分离，按公子量分成5个级别，用苯酚-硫酸比色法分别测定不同分子量范围多糖的糖含量，结果表明：它们占总糖的比例分别为：≥100000的占3．39％，30000～100000的占10．47％，10000～30000的占5．42％，6000-10000的占20．04％，≤6000的占60．68％．另外各级多糖均具有抗氧化活性，其中10000～30000和≤6000分子量的白术多糖对超氧阴离子自由基（O2^-·）和羟基自由基（·OH）具有极强的消除作用．
Atractylodes macrocephala polysaccharides were ultrafiltered by an ultrafiltration membrane separation unit, and they were divided into 5 levels in accordance with molecular weight （MW）. The contents of polysaccharides for different MW were determined with the phenol-sulfuric acid method. The result showed that the proportion of the polysaccharides with different MW in the total polysaccharides were as follows： 3.39% for MW above 100 000, 10.47% for MW between 30 000 and 100 000, 5.42% for MW between 10 000 and 30 000, 20.04% for MW between 6 000 and 10 000, and 60. 68% for MW less than 6 000. Determination of antioxidant activity demonstrated that polysaceharides of different MW all had antioxidant activity, and polysaccharides with an MW between 10 000 and 30 000 and less than 6 000, in partitular, had a very strong seavenging action on O2^- · and · OH.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期79-82,共4页
Journal of Southwest University(Natural Science Edition)
基金
广东省科技计划资助项目(2006B20301052)
关键词
白术多糖
超滤
自由基
清除作用
抗氧化作用
Atractylodes macrocephala Koidz polysaccharides
ultrafiltration
free radicals
scavenging action
antioxidant action
