摘要
目的:探讨D-氨基葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖(CO-PADG)对人食管癌Eca-109细胞增殖和凋亡的影响及其作用机制。方法:体外培养Eca-109细胞,CO-PADG作用Eca-109细胞不同时间;倒置显微镜下观察细胞形态学变化;MTT法检测不同时间点细胞增殖抑制率;流式细胞术检测COPADG作用后Eca-109细胞内Bcl-2及WTp53蛋白表达的变化;AnnexinV/PI染色结合流式细胞术检测COPADG作用后Eca-109细胞凋亡率。结果:COPADG作用显著增加Eca-109细胞增殖抑制率及凋亡率;COPADG作用使Eca-109细胞内Bcl-2蛋白表达明显下调而WTp53蛋白表达无变化。结论:COPADG显著抑制Eca-109细胞增殖并诱导细胞凋亡发生,细胞内Bcl-2蛋白表达下调,并可能通过该机制诱导细胞凋亡的发生。
Objective：To observe the effect of D - glucosamine[2 - （3 - carboxy - 1 - oxopropyl）amino -2 -de- oxy -D -Glucose, COPADG] on cell proliferation and apoptosis and expression of Bcl -2 protein and WTp53 protein in human esophageal cancer Eca - 109 cells and discuss the possible mechanisms. Methods： Eca - 109 cells were cultured in vitro by using RPMI - 1640 and calf serum,then were exposured to COPADG at 0.03mmol/L concentrations for different time. Cell growth inhibitory rate was detected by MTT colofimetric assay;The cell morphological changes were observed by inverted phase contrast microscopy. Expression of Bcl -2 protein and WTp53 protein was detected by using flow cytometry and apoptosis rate was analyzed by using Annexin V/PI fluorescence staining togeth er with flow cytometry. Results：COPADG could significantly inhibit the proliferation of Eca - 109 cells and induce them to apoptosis,and also decrease expression of Bcl -2 protein but expression of WTp53 protein didn＇t show any defferences. Conclusion：COPADG has significant antiproliferative and proapoptotic effect on human esophageal cancer cell line Eca -109, and downregnlates expression of Bcl -2 protein, which may play a very important role in apoptosis of Eca - 109 induced by COPADG.
出处
《现代肿瘤医学》
CAS
2009年第5期808-811,共4页
Journal of Modern Oncology
基金
中科院西部之光资助项目(200524号)
