摘要
研究了5种非离子型表面活性剂(BSA,Tween-20,Tween-80,PEG-4000,PEG-6000)促进麦草同步糖化发酵的效果.结果表明,5种表面活性剂均能促进麦草同步糖化发酵,以Tween-20效果最为显著.反应体系中添加Tween-20可降低酶用量而保持乙醇浓度基本相同.在pH5.0、温度37℃、底物浓度50g/L及Celluclast1.5l用量25FPU/g、Novozym188用量15IU/g的反应体系中,添加0.03g/gTween-20,反应72h,乙醇浓度达到18.7g/L,比未添加表面活性剂的体系提高了14.0%,反应时间缩短了12h.
Five different non-ionic surfactants, BSA, Tween-20, Tween-80, PEG-4000 and PEG-6000, were evaluated for their ability to enhance simultaneous saccharification and fermentation （SSF） of wheat straw. The results indicated that all non-ionic surfactants tested improved the SSF of wheat straw, and Tween-20 was the most effective. Addition of Tween-20 decreased the enzyme loading while retaining the same ethanol concentration. The ethanol concentration of 18.7 g/L was achieved at a substrate concentration of 50 g/L at 37 ℃ and pH 5.0 using cellulase mixture （25 FPU/g of substrate for CeUuclast 1.5 1 and 15 IU/g for Novozym 188） for 72 h. Compared with the reference run without Tween-20, the ethanol concentration was increased by 14.0% whereas the residence time reduced by 12 h.
出处
《过程工程学报》
CAS
CSCD
北大核心
2009年第2期355-359,共5页
The Chinese Journal of Process Engineering
基金
天津市科技攻关计划基金资助项目(编号:05YFGPGX05700)
关键词
麦草
表面活性剂
同步糖化发酵
乙醇
wheat straw
surfactant
simultaneous saccharification and fermentation
ethanol
