摘要
目的评价全光纤光学相干断层成像（全光纤OCT）检出早期牙釉质人工龋的敏感性。方法在新鲜拔除的7颗离体人恒牙釉质光滑面制备2mm×3mm实验窗．选用pH4．5的化学酸蚀液在开窗区制备0～120h早期脱矿人工龋模型．采用自行研发的口腔全光纤OCT系统检测离体人恒牙早期牙釉质人工龋，以同一牙齿样本的显微数码摄影和偏光显微镜检测作为对照．比较和检验OCT有效检出早期龋变的敏感性和可行性。结果全光纤口腔OCT装置可早期准确检测到脱矿12h以内牙釉质表面组织学水平的微观改变，早于裸眼和临床显微摄影的观察结果：开窗区釉质表面完整度随脱矿作用时间出现递减变化；而釉质脱矿深度随脱矿作用时间而出现递增性变化，不同时段脱矿深度的OCT量化数据与偏光显微镜检出结果相吻合．统计学分析结果表明2者间无显著性差异（P〉0．05），证实OCT可早期、敏感、精确地检出组织学水平的早期牙釉质人工龋。结论全光纤口腔OCT系统可无创准确检出早期牙釉质表面和表层下人工龋变．获得的龋变深度量化测量数据与偏光显微镜下组织学水平的测量结果高度相关。系统进一步完善后有望作为一种新型光学手段用于口腔临床龋病的早期诊断、量化监控以及干预效果的有效评估。
Objective To investigate the sensibility of All-fiber optical coherence tomography （All-fiber- OCT） system for early quantification detecting of artificial caries in vitro. Methods Prepared 2 mm×3 mm experimental windows in adamant slippery surfaces of 7 fresh uprooted permanent teeth, demineralized the teeth surfaces in the windows area to creat early stage of artificial caries mould, by aciding the experimental teeth surfaces of 0- 120 hours with demineralized liquid （pH4.5） in vitro. Detected the demineralized changes on the experimental teeth surfaces by dental All-fiber- OCT system, which were newly developed by our research group, and compared the detecting results with clinical digital photomicrography （DPM） and polarimicroscopy （PMS） on the same tooth sample, to checkout the sensibility and feasibility of All-fiber-OCT for early quantification detecting of artificial caries in vitro. Results Our All-fiber- OCT system could accurately detect surface demineralization changes on the experimental windows of artificial dental caries as early as 12 h aciding treatment, earlier than the survey results obtained by DPM;the surface Integrity of the experimental windows decrease progressively with the increasing Aciding hours, while the demineralized depth increased progressively with the increasing aciding hours. It was shown that quantification detecting data by OCT has high correlation to those by PMS, There are no significant differences between data obtained from OCT and PMS（P〉0.05）. Conclusion All-fiber-OCT system could accurately detect early artificial dental caries without trauma in our model. Quantification detecting data of demineralized depth by OCT has high correlation to those by PMS. It has shown exact detection efficacy on enamel early caries in our experiment and might become a potential new optical detecting technique for early diagnosis, quantification process monitoring and early intervention of dental caries in vivo after the All-fiber OCT system further being consummated.
出处
《国际生物医学工程杂志》
CAS
北大核心
2009年第2期65-69,I0001,共6页
International Journal of Biomedical Engineering
基金
国家自然科学基金资助项目（30770597）志谢 本研究得到了2007年度国家自然科学基金的经费资助
全光纤OCT系统研制得到了美国General Photonics公司的光学元器件援助,天津市口腔医院和天津大学2个合作单位的各级领导给予了该课题全方位大力支持.研究团队全体同仁精诚合作勇于探索求新,付出了卓有成效的努力.此外,本研究的牙齿标本制样得到了成广泽老师的倾心指导,在此一并致谢
关键词
光学相干断层成像
全光纤
牙釉质龋
人工龋模型
早期检出
量化
敏感性
Optical coherence tomography
All-fiber
Enamel carie
Artificial caries modeling
Early detection
Quantification
Sensibility
