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急性有氧运动对C57BL／6小鼠骨骼肌PPARδ表达和相关酶活性影响的研究

Study on Expression of PPARδ and Activity of Some Interrelated Enzymes after Acute Aerobic Exercise in C57BL/6 Mice Skeletal Muscle
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摘要 目的:观察急性有氧运动对PPARδ表达和相关代谢酶活性的影响,以研讨耐力训练使骨骼肌有氧代谢能力产生适应性变化的机制。方法:6周龄雄性C57BL/6小鼠,体重为15±2g,对照组8只,运动组32只。运动组以20m/min速度在跑台上运动120min,并在运动后的即刻、1h、3h和6h分别处死8只小鼠。采用RT-PCR和Western blot方法检测骨骼肌中的PPARδ、CPT-1和BoAcDH的表达。结果:与对照组相比,运动组PPARδ的mRNA和蛋白表达量分别增加了2.3倍和1.0倍(P<0.05),CPT-1和BoAcDH的蛋白表达分别提高了1.3倍和1.9倍。结论:急性有氧运动诱导了PPARδ的过量表达,从而调控脂肪酸氧化相关酶的活性,促进骨骼肌有氧代谢能力增强,成为长期训练使肌肉产生适应性变化的基础。 Objective： Observing the influence of acute aerobic exercise on the expression of PPARδ and activity of some interrelated enzymes in order to study the mechanism of aerobics causing skeletal muscle biologically adaptive change. Method： male mice （C57BL/6）, 6-week- old, 15±2g in weight, 8 in control group and 32 in exercise group. The mice in exercise group run 120mins at a speed of 20m/min, and they were killed immediately, an hour, three hours and six hours after exercises respectively. RT-PCR and Western blot are applied to examine the expression of PPARδ, CPT-1 and BoAcDH in skeletal muscles. Results： In comparison with control group, the expression of mRNA and protein in PPARδ has increased respectively by 2. 3 times and 1 time（P〈0.05）in the exercise group, and the expression of protein in CPT- land BoAcDH has increased respectively by 1.3 and 1.9 times. Conclusion： Acute aerobic exercise induced the excessive expression of PPARδ, thus adjusted the activity of interrelated enzyme in oxidizing fatty acid and enhanced the increase in the ability of aerobic metabolism to become the change basis of adaptability caused by long-term training.
作者 席翼 温含 武雅琼 苏丽 姜宁 牛燕媚 苑红 傅力
机构地区 天津体育学院运动人体科学系 深圳大学体育系 河南省体育科学研究所 秦皇岛水运卫生学校
出处 《中国体育科技》 CSSCI 北大核心 2009年第3期137-140,共4页 China Sport Science and Technology
基金 国家自然科学基金资助项目(30570898) 天津市重大攻关项目(05YFGDSF02100)
关键词 急性有氧运动 PPARΔ MRNA和蛋白表达 酶活性 鼠 动物实验 acute aerobic exercise PPARS expression of rnRNA and protein activity of enzyme mice animal experiment
分类号 G804.7 [文化科学—运动人体科学]
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参考文献12

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二级参考文献23

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中国体育科技
2009年 第3期

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