摘要
目的观察不同剂量三氯苯达唑治疗大鼠斯氏狸殖吸虫童虫感染的疗效,探讨其量效关系和最适剂量。方法60只SD大鼠随机分成实验组(A、B、C)和对照组D,每组15只大鼠。将斯氏狸殖吸虫囊蚴经腹腔注射感染各组大鼠,感染后第39dA、B、C组大鼠分别给予三氯苯达唑50、100和200mg/(kg·d)×4d治疗,治疗结束后第1、15和22d剖杀大鼠,回收虫体。并计数,计算检虫率及减虫率;称虫体湿重,并与对照组比较,进行统计学分析。结果治疗结束后第1d,检虫率和虫体湿重组间比较差异均无统计学意义(P均>0.05);治疗结束后第15d,中、高剂量组检虫率、减虫率及虫体湿重与对照组比较,差异有统计学意义,低、中、高剂量组间比较,差异有统计学意义(P均<0.01),而低剂量组与对照组比较,差异无统计学意义(P>0.05);治疗结束后第22d,检虫率、减虫率及虫体湿重组间比较,差异均有统计学意义(P均<0.01)。结论三氯苯达唑对大鼠斯氏狸殖吸虫童虫感染疗效显著,但其杀虫过程缓慢,随着治疗后时间延长,疗效逐渐提高,其呈明显的剂量效应关系,最适治疗剂量为200mg/(kg·d)左右。
Objective To observe the therapeutic effect of different doses of triclabendazole in rats infected with the juvenile worms of Pagumogonimus skrjabini, to investigate the regularity of the dose-effect relationship and to probe into the best dosage of triclabendazole. Methods 60 rats were randomly divided into four groups： experimental group A, experimental group B, experimental group C and control group D, with 15 rats in each group. The 60 rats were each in- jected through the abdominal cavity with the metacercariae of P. skrjabini. Rats of the experimental groups were treated with different doses of triclabendazole on the thirty-ninth day after infection. Rats were dissected on the first, fifteenth and twenty-second day after finishing the treatment to recover the worms, count the number of worms recovered, calculate the worm recovered rate and worm reduction rate, and measuring the wet weight of the worms recovered in comparison with the control group. Data were analyzed with SPSSll. 5 statistical software. Results Based on the worm recovered rate and the worm reduction rate on the first day after finishing the treatment, there was no significant differences between the rats in the control group and those in the low, middle and high-dose groups（P〉0.05）. On the fifteenth day after finishing the treatment, there were significant differences between the middle and high-dose groups, and there were significant differences between the low, middle and high-dose groups（P〈0.01） although there were no significant differ- ences between rats in the low-dose and control groups（P〉0.05）. On the twenty-second day after finishing the treatment, there were significant differences between rats in the control, low, middle and high-dose groups（P〈0.01）. Wet weight measurement of the worms had results that were similar to that of the worm recovery rate and worm reduction rate. Conclusion Triclabendazole has a remarkable therapeutic effect on rats infected with the juvenile worms of P. skrjabini, but the insecticidal course is tardigrade and the worms can be killed gradually. The therapeutic effect enhances gradually with time after treatment. Triclabendazole has a remarkable dose-effect relationship on rats infected with the juvenile worms of P. skrjabini, and the best dose of triclabendazole to treat rats infected with the juvenile worms of P. skrjabini is about 200 mg/（kg · d）.
出处
《中国病原生物学杂志》
CSCD
2009年第4期287-289,292,共4页
Journal of Pathogen Biology
关键词
三氯苯达唑
斯氏狸殖吸虫
剂量效应关系
大鼠
Triclabendazole
Pagumogonimus skrjabini
dose-effect relationship
rats
