摘要
我们在神经移植的研究过程中观察到被移植的中枢神经元能从蛛网膜下腔迁入脊髓和大脑皮层。这一新观察为脊髓和脑浅层大范围神经元缺损时的无损伤神经元引入和大范围去神经区域的神经再支配提供了一种颇具吸引力的可能性。实验动物选用Wistar和S．D．大鼠，将含有胚胎中枢单胺或精氨酸血管加压素（AVP）能神经元的细胞悬浮液或组织块移植到被横断的脊髓或未被横断的脊髓和脑的蛛网膜下腔内。动物分别在移植的同时切断脊髓；在移植前或后一个月切断脊髓；以及不切断脊髓的单独移植。这些动物再饲养1个月或9个月后用免疫组织化学方法跟踪移植神经元的迁移。结果显示：经移植后，在移植区的脊髓内出现数个到数百个不等的TH或AVP兔疫阳性反应胞体和纤维；移植在脑部蛛网膜下腔的5－HT能神经元迁入大脑皮层。这些神经元生长良好，显示出它们的适应和长期存活的能力。但未发现有分散的神经元能在蛛网膜下腔内长期存活。若以组织块为移植物，则可见残留的移植物像"小瘤"附着在脊髓表面。这一现象偶尔也出现在移植细胞悬浮液的动物，其原因可能是悬浮液内的细胞过分集中于蛛网膜下腔的某一局部，但"小瘤"和脊髓间已无软膜可见，免疫阳性反应细胞可同时存在于"小瘤"和相邻的脊髓内；"小瘤"
In the studies of neural transplantation,Chen and his colleagues observed tha the grafted central neurons could migrate from the subarachnoid space into the spinal cord and cerebral cortex.This finding promises an attractive possibility that a lot of neurons might be introdced into a long distance of the spinal cord and the extensive superficial layer of the brain which has suffered neuronal loss and thus might reinnervate a wide range of denervated area.Female rats were used in the study.Neural tissues or cell suspension containing fetal monoaminergic or arginine vasopressin(AVP)neurons were implanted into the subarachnoid space of the transected spinal cord or the normal brain and spinal cold.The rats were treated in different ways:some were grafted and the spinal cord was cut at the same time;grafted one month later or before the spinal cord was cut;grafted but the brain and spinal cord remained intact.Immununohistochemical method was used to monitor the grafted neurons after these rats survived for one or nine months.The results showed that there were a few to hundreds of 5-HT,TH or AVP-immunoreactive neurons and fibers found in the spinal cord or cerebral cortex near the grafted region.These neurons grew well and displayed their capacity of adaptability and long-time survival in the host CNS.No neurons,however,were found in the subarachnoid space of the grafed rats which had survived for another month or longer.On the other side,grafted with tissue block,it was found that transplants left in the grafted region grew as a'nodule'attaching to the surface of the spinal cord.The'nodule'was also found occasionally when the cell suspension was centered heavily at one region of the subarachnoid space.In both cases,the pia mater between them disappeared.Immunoreactive neurons were found in the'nodule'and neighboring spinal cord,some fibers from the neurons in the 'nodule'extended into the spinal cord.It is supposed that a part of cells in the suspension died after grafted in the subarachnoid space and released hydrolases which injured the near pia mater by hydrolysis.As a result,some neurons in the suspension had a chance to ingrate into the spinal coed and cereball cortex. After the transPlant in thesubarachnoid space was gradually cleaned out,the enzymatic hydrolysis to the pia mater became weaker and finally stopped,the lesioned pia mater was gradually repaired.If the transplant was not cleared timely,the pia mater could not withstand the persistent hydrolysis and collapsed finally,leading the transplant to be fused with the host CNS.Supported by tha National Netural Science Foundation of China(No.3937047)Presant address:Department of Histology and Embryology,Shanghai Medical Uniersity,Shanghai 200032Corresponding author
出处
《生理学报》
CAS
CSCD
北大核心
1998年第1期28-36,共9页
Acta Physiologica Sinica
基金
国家自然科学基金
关键词
中枢神经元
蛛网膜下腔
脊髓
大脑皮层
神经移植
central neuron
subarachnoid space
transplantation
migration
spinal cord and cerebral cortex
