摘要
目的探讨异丙酚对大鼠空间学习记忆及海马CA1区突触结构的影响。方法雄性Wistar大鼠36只,日龄18～21d,体重40～60g,随机分为3组,每组12只,分别腹腔注射0.9%生理盐水10mL/kg(A组)、异丙酚50mg/kg(B组)或异丙酚200mg/kg(C组),连续用药5d。每天均采用Morris水迷宫实验测试大鼠空间学习记忆能力。第5天测试结束后处死大鼠取脑分离海马组织,应用透射电镜及图象分析方法观察并测定海马CA1区突触结构的形态参数。结果与第1天比较,各组第4,5天逃避潜伏期明显缩短(P<0.05);与A组比较,B,C组第4,5天逃避潜伏期延长(P<0.05);与B组比较,C组第4,5天逃避潜伏期延长(P<0.05)。与A组比较,B,C组大鼠海马CA1区突触结构的突触后致密物质厚度和突触活性区长度变小,突触间隙变窄(P<0.05);与B组比较,C组突触后致密物质厚度和突触活性区长度改变更为明显(P<0.05)。结论异丙酚可引起幼年大鼠的空间学习记忆能力降低,其机制可能与其破坏海马神经元突触结构有关。
Objective To explore the effects of different doses of propofol on spatial learning and memory and synaptie structures in hippoeampal CA1 area of rats. Methods Thirty - six male Wistar rats were randomly divided into three groups： control（ group A） , 50mg/kg propofol （group B）, 200mg/kg propofol （group C ）. Normal saline or propofol was injected for 5 days. All rats were tested for spatial learning and memory in Morris water maze （MWM） every day. After the test, the uhrastructural features of hippocampal synapses were observed with transmission electron microscope. The presynaptic membrane, the thickness of post - synaptie density （ PSD）, the length of active zone and the width of synaptic cleft were quantitatively described. Results The latency to escape from water in MWM was shortened in all of groups after three days of training. In 4th or 5th day, the latency in group B or C was longer than that in group A and the latency in group C was longer than that in group B. The thickness of PSD, the length of active zone and the width of synaptic cleft all decreased in group B or C. Conclusion Propofol may impair spatial learning and memory function by destroying synaptie structures.
出处
《广东医学》
CAS
CSCD
北大核心
2009年第5期691-693,共3页
Guangdong Medical Journal
基金
广西医疗卫生重点科研项目(编号:重200740)
广西科学基金资助项目(编号:桂科自0861004)
关键词
异丙酚
学习
记忆
海马
突触
propofol
learning
memory
hippocampus
synapse
