摘要
[目的]提取并测定五味子藤茎中多糖的含量。[方法]采用超声技术提取、苯酚一硫酸法测定含量，以葡萄糖（105℃干燥至恒重）为对照品。[结果]在10．125～71．064μg/ml的范围内，样品的浓度与吸光度呈良好线性关系，线性回归方程为A=0．0094C＋0．0029（r=0．9998），加样回收率为99．93％（n=5，RSD=0．06％），五味子藤茎中多糖平均含量为5．27％。[结论]五味子藤茎中多糖含量较高，具有较高的药用价值和开发前景。
[ Objective] The aim of the research was to extract and determine the content of polysaccharide in the rattan stem of Schisadlra chinensis. [ Method] Polysaccharide was extracted by ultrasonic technology and the content was determined by phenol-sulfuric acid method, with glucose as the control substance. [Result] The sanole concentration has a good linear relationship with the absorbance within the range of 10.125 - 71.064 w,/ml.And the linear regression equation was A =0.009 4C ＋0.002 9（r =0.999 8）. The average recovery was 99.9＂3% （n = 5,RSD = 0106% ） and the average content of polysaccharide was 5.27%. [ Conclusion] The content of polysaccharide in the rattan stem of Schisandra chinensis was high, and it had higher medical value and development foreground.
出处
《安徽农业科学》
CAS
北大核心
2009年第15期6990-6991,共2页
Journal of Anhui Agricultural Sciences
基金
吉林省教育厅"十一五"规划项目(吉教科合字[2008]第216号)
关键词
五味子藤茎
多糖
苯酚-硫酸法
含量测定
Rattan stem of Schisandra chinensis
Polysaccharide
Phenol-sulfuric acid method
Content determination
