摘要
目的:探讨丹参对豚鼠耳蜗缺血再灌注损伤(Ischemia reperfusion injuryI/RI)后细胞凋亡及Fas蛋白表达的影响。方法:健康豚鼠72只,雌雄不拘。随机分成正常组、假手术组、丹参干预假手术组、模型组和干预组(术前腹腔注射丹参注射液,每天一次共7d),后两组各在缺血30min、再灌注6h及24h取材。用组织化学方法观察缺血及再灌注不同时间段耳蜗各部位的组织学改变;用免疫组织化学法检测内耳Fas的表达;用原位凋亡法(TUNEL法)检测内耳细胞凋亡情况。结果:正常组、假手术组、丹参干预假手术组内耳罕见调亡细胞,Fas为弱阳性表达;模型组Corti器、血管纹、螺旋神经节在缺血及再灌注每个时间点均有细胞凋亡,Fas为阳性表达,与正常组比较有统计学意义(P<0.05);干预组Corti器、血管纹、螺旋神经节在缺血及再灌注每个时间点均有细胞凋亡,Fas为阳性表达,程度较模型组减弱,与模型组比较有统计学意义(P<0.05)。结论:Fas参与了耳蜗I/RI后细胞凋亡的发生,丹参可能通过抑制Fas蛋白的表达使凋亡细胞减少而对I/RI后的耳蜗起保护作用。
Objective： To investigate the effects of Salvia Miltiorrhiza on the cell apoptosis and the expression of apoptosis-associated Fas protein in cochlea of guinea pigs encountered ischemia-reperfusion injury. Mothods： 72 healthy guinea pigs were divided into the normal group, negative control group, negative control group treated with Salvia Miltiorrhiza, model group, and drug treated group given Salvia Miltiorrhiza everyday by intraperitoneal injection for 7 days before onset of ischemia. Animals of the last two groups were taken samples 30 minutes after ischemia, 6 hours after reperfusion and 24 hours after reperfusion. The histochemical features of cochlea were observed by HE staining. The expression of Fas protein in cochlea was studied by immunohistochemisty, while labeling ratio of Fas expression was studied by image analysis. The cell apoptosis was studied by TUNEL. Results： There was no apoptotic cell was found in the normal and control groups, with the weakly positive expression of Fas protein, however, obvious cell apoptosis presented in ischemical and the reperfused groups, with the strong expression of Fas. The numbers of apoptotic cells in the drug groups were lesser than that of others. Conclusions： Salvia Miltiorrhiza could inhibit cell apoptosis by down-regulating the expression of Fas protein, which may be one of mechanisms of neuroprotective effects of Salvia Miltiorrhiza.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2009年第3期302-305,共4页
Chinese Journal of Clinical Anatomy
基金
福建省卫生厅科研基金(2006-1-36)
关键词
豚鼠
耳蜗
丹参
缺血再灌注损伤
FAS蛋白
细胞凋亡
guinea pig
cochlea
Salvia Miltiorrhiza
ischemia-reperfusion injury
Fas protein
apoptosis
