摘要
以金黄色葡萄球菌和大肠杆菌为实验对象,采用抑菌环实验研究了儿茶素的最小抑菌浓度,并对儿茶素作为抑菌剂的稳定性作出了评价,同时还研究了儿茶素可能存在的抑菌机理。结果显示,儿茶素对金黄色葡萄球菌和大肠杆菌的最小抑菌浓度分别为0·32g/L和1·25g/L;儿茶素的抑菌效果较大程度地受温度和pH的影响,较小程度受紫外照射的影响;儿茶素会抑制金黄色葡萄球菌和大肠杆菌的对数生长期,增加细菌细胞膜的通透性,造成细菌胞内蛋白质和糖类物质的渗漏,使细菌代谢发生紊乱,儿茶素的抑菌作用不是通过抑制呼吸代谢而引起的。
The minimal bacteriostasic concentration, stability and mechanism of catechins toward S.aureaus and E.coli were studied.The result showed that the MIC of catechins on S.aureaus and E.coli respectively were 0.32g/L and L25g/L.Temperature and pH influenced the bacteriostasic activity of catechins in large extent and UV irradiation had a little influence on the bacteriostasic activity of catechins.This experiment proved that the antibacterial mechanisms of catechins were to restrain logarithmic growth of S.aureaus and Ecoli, to increase the permeability of bacterial cell membrane（ increase the leakage of protein and sugar）.And then this experiment proved that restraining breath metabolism was not the antibacterial mechanism.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第5期89-92,共4页
Science and Technology of Food Industry
基金
国家"十一五"科技支撑计划项目(2006BAD06B01)
关键词
儿茶素
抑菌
机理
catechins
bacteriostasic
mechanism
