摘要
系统研究了压力、温度、静态萃取时间、动态萃取量、改性剂加入量等因素对超临界流体萃取（ＳＦＥ）中药川芎中藁本内酯的影响，确定最佳萃取条件为：压力２７６ＭＰａ，温度４０℃，静态萃取时间３ｍｉｎ，动态萃取量７ｍｌ，改性剂加入量０１ｍｌ。并对超临界流体萃取的收集方法作了研究，发现固液收集法在收集效率和精密度方面比溶剂收集法效果好。并用离线的ＳＦＥＲＰＨＰＬＣ对川芎药材中藁本内酯的含量进行了测定。
In this study, a systematic method was used to optimize the supercritical fluid extraction (SFE) of ligustilide in Ligusticum chuanxiong Hort. Overall five variables (pressure, temperature, static extracting time, modifier concentration and CO2 dynamic extracting volume) were considered. To reduce the loss of volatile substance, a solidliquid trap was developed for SFE collection. Comparisons were made on the solvent trap for SFE. The extracts were analyzed by high performance liquid chromatography (HPLC) with ultraviolet absorbance detection. The recoveries of 9934% (RSD 170%) and 9211% (RSD 572%) were achieved for solidliquid trap and solvent trap, respectively.SFE conditions: temperature 40℃, pressure 276 MPa, static extraction 3 min, dynamic extraction 7 ml and 01 ml CHCl3 as modifier.HPLC conditions: The HPLC column (39 mm×150 mm)was packed with NovaPak C18 (4 μm). Naphthalene was used as internal standard. The mobile phase was methanol—10% isopropyl alcohol solution (53∶47 v/v). The detection wavelength was 280 nm and the flow rate was 08 ml·min-1.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第6期457-460,共4页
Acta Pharmaceutica Sinica
关键词
超临界萃取
川芎
藁本内酯
高效液相色谱
HPLC
Supercritical fluid extraction
Solidliquid trap
Ligustilide
Ligusticum chuanxiong Hort
