摘要
目的:研究雌激素对抑郁症大鼠行为学及缰核内去甲肾上腺素含量的影响,探讨雌激素在抑郁症发病中的作用。方法:40只雌性Wistar大鼠经敞箱试验初筛后,将行为学评分相近的30只随机分为假手术抑郁症模型组(Sham)、去卵巢抑郁症模型组(OVX)和去卵巢抑郁症雌二醇干预组(OV/ER)。将大鼠卵巢摘除后,采用慢性应激刺激制作抑郁症模型,自刺激第3天起对OV/ER组进行雌激素干预。运用敞箱试验和强迫游泳试验观察大鼠行为学的改变,应用高效液相色谱法检测其缰核内去甲肾上腺素(NE)含量的变化。结果:慢性应激第21天时,敞箱试验显示,OV/ER组大鼠水平运动和垂直运动与OVX组比较行为活动明显增加(P<0.05),OV/ER组中央格停留不动时间与OVX组比较明显减少(P<0.05);强迫游泳试验显示,OV/ER组水中不动时间明显低于OVX组(P<0.05),OV/ER组上窜时间明显高于OVX组(P<0.05)。OV/ER组缰核内NE含量与OVX组比较明显升高(P<0.05)。结论:缰核可作为雌激素作用的靶点,参与其介导的抗抑郁作用。
Objective To observe the effects of estrogen on the behavior and the norepinephrine level in the habenular nucleus of depressive rats, and explore the mechanism of estrogen involved in the process of antidepression. Methods Thirty rats were chosen from forty male Wistar rats, which had the similar scores in open field test. The depression models of rats were established by chronic mild stress. Open field test and forced swimming test were performed to detect the behavioral changes between rats with ovary excision （OVX） and with estradiol-treated ovary excision （OV/ER）. High performance liquid chromatography （HPLC） was used to observe the changes of norepinephrine level in habenular nucleus. Results After 21-day chronic mild stress, the open field test showed that the vertical motion and cross motion in OV/ER group were increased （P〈0.05） and the immobile time in OV/ER group was decreased （P〈0.05）, when compared with those in OVX group. Forced swimming test demonstrated that the climbing time in OV/ER group was higher than that in OVX group （P〈0.05） ; while the immobile time in OV/ER group was obviously lower than that in OVX group （P〈0. 05）. The norepinephrine level in the habenular nucleus in OV/ER group was obviously increased compared with OVX group （P〈0.05）. Conclusion The habenular nucleus is a target of estrogen in central nervous system, which can take part in the antidepressive effect induced by estrogen.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2009年第3期397-399,共3页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30571724)
关键词
抑郁症
缰核
雌二醇
去甲肾上腺素
depression
estradiol
habenular nucleus
norepinephrine
