摘要
目的:对栀子果实、果仁、果皮中二萜色素类成分进行比较研究。方法:HPLC法同时测定3个主要成分(crocin1,crocin 2,crocin 3)含量,kromasil C18柱,流动相为甲醇-乙腈-0.3%甲酸水不同梯度洗脱,检测波长440 nm,流速1.0 mL.min-1,柱温35℃。结果:3个成分在测定范围内线性关系较好(r>0.999 5),平均回收率分别为97.50%,98.76%,97.67%。栀子果实不同部位中均含有3种二萜色素类成分,且含量有较大差异。结论:3种成分以栀子果仁中含量最高,为揭示栀子果仁。
Objective： To compare the contents of diterpenoid pigments among the fruit, seed, and pericarp from Gardenia jasminoides. Method： The separation of Crocin 1, Crocin 2, Crocin 3 were carriedout simultaneously on a Kromasil C18 column at 35 ℃ with the methanol-acetonitrile-0. 3 % formic acid anhydrous in gradient elution as the mobile phrases. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL.min^-1. Result： The obtained linearity of the three components was better over 0. 999 5 and the average recoveries were 97.50%, 98. 76%, 97.67%, respectively. The results exhibited the remarkable variation among the different parts of the fruit. Conclusion： The contents of three constituents in seed were higher than in pericarp.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第11期1395-1397,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30672666)
关键词
栀子
果仁
果皮
二萜色素
HPLC
含量测定
Gardenia jasminoides
seed
pericarp
diterpenoid pigments
HPLC
Content determination
