摘要
目的:观察功能性电刺激(FES)治疗对急性脑梗死大鼠运动功能和缺血半影区微管相关蛋白-2(MAP-2)表达的影响。方法:制作急性大脑中动脉栓塞模型大鼠。随机分为假手术组、FES安慰治疗组和FES治疗组共三组,每组又分为治疗0d、3d、7d、14d四个亚组,每亚组共6只,术后3天开始FES治疗。FES治疗引起瘫痪侧伸腕伸指动作,每天1次,每次10min。在治疗前和治疗后的各个时间点采用网屏试验评定运动功能,评定后取材,应用免疫组化技术观察半影区MAP-2变化。结果:治疗7d和14d后,FES治疗组的运动功能较安慰治疗组明显改善(P<0.05)、MAP-2阳性细胞数和光密度值明显增加(P<0.01)。结论:FES治疗可以改善急性脑梗死大鼠的运动功能,并增强脑梗死周围缺血半影区MAP-2的蛋白表达,增强脑的可塑性。
Objective： To observe the effects of functional electrical stimulation （FES） on motor function and the expression of microtube-associated protein 2 （MAP-2） in the penumbra field of the rats with acute cerebral infarction. Method：Acute cerebral infarction was made by means of middle cerebral artery occlusion. Rats were randomly divided into sham-operated, FES placebo-treatment and FES treatment groups. Each group was divided into 0-day, 3-day, 7-day and 14-day subgroups and 6 rats for each subgroup. FES treatment to induce wrist and fingers extension was carried out 3 days after operation and was performed once daily, 10 minutes each time. The motor function and protein expression of MAP-2 were observed before treatment and at each treatment points. Result：After treatment for 7 days and 14 days, the motor function of FES treatment group was significantly improved, comparing with that of FES sham-treatment group （P〈0.05）, and the MAP-2 labeled cells and expression were also significantly enhanced comparing with that of the FES sham-treatment group （P〈0.01）. Conclusion：FES treatment can improve motor function and enhance the expression of MAP-2 in the penumbra field of the rats with acute cerebral infarction.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2009年第6期505-508,I0002,共5页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金项目(30772304)
广东省自然科学基金项目(8451008901000885)
关键词
功能性电刺激
脑梗死
运动功能
微管相关蛋白
functional electrical stimulation
cerebral infarction
motor function
microtube-associated protein
