摘要
目的:观察番石榴叶水煎液及其总黄酮抗轮状病毒作用,为寻求新的抗轮状病毒药物提供实验依据。方法:提取番石榴叶水煎液及其有效成分黄酮,用甲基噻唑基四唑(methyl thiazolyltetrazolium,MTT)法检测番石榴叶提取物作用于MA104细胞后的细胞存活率,求出其半数细胞毒性浓度(median toxic concentration,TC50)后,给予低于TC50不同浓度的药物对轮状病毒感染细胞进行干预,检测其对病毒感染细胞存活的影响,以确定番石榴叶水煎液及其总黄酮体外抗轮状病毒的作用。结果:番石榴叶水煎液及黄酮均有预防轮状病毒感染的作用,其用于预防时治疗指数(therapeutic index,TI)分别4.65和1.83。同时亦对病毒感染的MA104细胞具有治疗作用,两者用于治疗轮状病毒感染时TI分别为2.98和3.07。随着药物浓度的增加,治疗和预防时病毒抑制率显著升高,呈量效关系。结论:番石榴叶水煎液及其总黄酮具有良好的体外抗轮状病毒的作用。
Abstract： Objective： To investigate the anti-rotavirus effects of Psidium Guajava Leaves, so as to provide an experimental basis for searching a new efficacious drug for treatment of rotavirus-caused diar- rhea. Methods： The flavonoids and water decoction were extracted from Psidium Guajava Leaves, and added into MA104 cells. Afterwards, the cell viability was detected by methyl thiazolyl tetrazolium （MTT） assay, and median toxic concentration （TCs0） was calculated. Then different concentrations of the two components, which was less than TG50, were added into rotavirus infected cells to determine their anti-rotavirus effects in vitro. Results. Both water decoction and flavonoids from Psidium Guajaava Leaves had preventive effects against infection by rotavirus. For prophylactic use, the therapeutic index（TI）was 4. 65 and 1.83, respectively. Mean while, the two components had therapeutic effects on rotavirus infected MA104 cells. The TI for treatment of rotavirus infection was 2. 97 and 3.07, respectively. The virus inhibition ratio for prophylactic and treatment use significantly increased with the drug concentration increasing, showing a dose-dependent manner. Conclusion： The flavonoids and flavonoids from Psidium Guajava Leaves have strong anti rotavirus effects in vitro.
出处
《安徽中医学院学报》
CAS
2009年第3期44-47,共4页
Journal of Anhui Traditional Chinese Medical College
关键词
轮状病毒
番石榴叶
黄酮
MTT法
Rotavirus
Psidium Guajava Leaves
Flavonoids
Met hyl thiazolyl tetrazolium assay
