摘要
目的建立车前子的槲皮素、山柰酚、木犀草素、芹菜素含量测定方法。方法采用反相高效液相色谱(RP-HPLC)法,色谱柱为ZorbaxSB-C18柱(150mm×4.6mm,5μm);检测波长360nm,流动相为甲醇-0.2%磷酸(45∶55),柱温30℃,流速1.0mL/min。结果槲皮素、山柰酚、木犀草素、芹菜素的回归方程分别为Y=1504.412X+9.9756,Y=1991.745X+8.6051,Y=567.591X+2.5397,Y=1811.803X+0.3074;质量浓度线性范围分别是0.05522～0.1933μg/mL,0.0461～0.1613μg/mL,0.1255～0.4393μg/mL,0.0603～0.211μg/mL,r在0.9999～1.0000之间;槲皮素、山柰酚的加样回收率分别为92.87%和102.95%,RSD分别为0.28%和1.08%;样品分别含槲皮素、山柰酚、木犀草素、芹菜素0.81,0.634,0,0.08mg/g。结论RP-HPLC法分析车前子中黄酮化合物的含量,方法简便可行,重复性好,结果可靠。
Objective To develop the RP- HPLC method for the identification and determination of quercetin, kaempferol, luteolin, apigenin in Semen Plantaginis. Methods RP-HPLC was used. The column was Zorbax SB-C18 （150 mm×4.6 mm, 5 μm） column; the wave-length was 360 nm, the mobile phase was methanol-water（45 ： 55）, the flow rate was 1.0 mL/min and the column tempreration was 30℃. Results The regression equations of quercetin, kaempferol, luteolin, apigenin were Y = 1 504. 412 X ＋ 9. 975 6, Y = 1 991. 745 X ＋ 8. 605 1, Y = 567. 591 X ＋ 2. 539 7, Y = 1 811. 803 X ＋ 0. 307 4 respectively. The linear range were 0. 055 22 - 0. 193 3 μg/mL, 0. 046 1 - 0. 161 3 μg/mL, 0. 125 5 -0. 439 3 μg/mL, 0. 060 3 -0. 211 μg/mL respectively; the correlation coefficients 0. 999 9 - 1. 000 0; the recovery rates of quercetin, kaempferol were 92. 87%, 102.95% ; RSD was 0. 28% and 1.08% respectively. The sample contents of quercetin, kaempferol, luteolin, apigenin were 0. 81, 0. 634, 0, 0.08 mg/g respectively. Conclusion This optimized method is effective and suitable for analyzing the above flavonoids in Semen Plantaginis.
出处
《中国药业》
CAS
2009年第12期32-34,共3页
China Pharmaceuticals
