摘要
构建了针对鹅源副黏病毒(GPMV)NA-1株NP及L基因的RNA干扰质粒;将转染质粒36 h后的鸡胚成纤维细胞接种病毒,通过病毒滴度、实时荧光定量RT-PCR、细胞病变以及间接免疫荧光结果分析,表明均能抑制GPMV在CEF中复制和增殖。本试验对研究GPMV复制和抗病毒治疗提供了技术基础。
We designed plasmids that continuously expressing siRNAs targeting the GPMV NA-1 matrix protein NP and L gene. Virus titration,Real time RT-PCR,CPE and IFA indicated that NP-specific and L-specific siRNA can inhibit virus replication at 36 h post-virus infection. In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第6期705-709,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30571375,30771606)
