摘要
利用分离鉴定的鸡鲍氏和鸡痢疾志贺氏菌，分别经细菌培养、灭活、浓度调整等方法研制诊断用鸡志贺氏菌凝集抗原，并用鸡志贺氏菌疫苗免疫清洁级小白鼠研制鸡志贺氏菌标准血清。结果显示，制备细菌抗原的最佳培养基是1％葡萄糖营养琼脂，抗原最佳灭活温度和时间为121℃和120min，凝集抗原合适浓度为40亿／mL，最佳稀释液为pH7．2的0．5％石炭酸生理盐水。制备的鸡鲍氏和鸡痢疾志贺氏菌阳性血清经效价测定分别可达8log2和10log2，阴性血清与鸡志贺氏菌凝集抗原作用无凝集反应发生。通过研究同时确定了平板凝集试验反应的合适温度为20～35℃，结果判定时间为3～5min。研制的凝集抗原及其标准血清具有特异性强、稳定性好、使用方法简便、便于保存和检测结果准确可靠等优点，便于在基层生产单位推广应用。
In order to diagnosis Shigella from chicken with the serological method accurately and rapidly, the ag- glutinating antigen from the isolated and identified of S. boydii and S. dysenteriae was prepared through bacterial cuhure,inactivation and concentration adjustment. Chicken Shigella standard serum was developed by vaccinating the clean level mice with inactivated vaccine. The results showed that the optimal conditions for shigellae culture was 1% glucose nutrient agar, the temperature and time to inactivate antigen were 121 ℃ and 120 min,antigen concentration was 40 billion per millilitre, the proper diluent was pHT. 2 0.5% carbolic acid-NaC1. Preparation of S. boydii and S. dysenteriae positive serum titer were 8 log2 and 10 log2, respectively,negative serum with agglutinating antigen was no agglutination. The proper reaction temperature and time were 20-35 ℃ and 3-5 minutes. The agglutinating an- tigen and standard serum had strong specificity, high stability,and easy preservation. This method was simple,accu- rate and reliable ,and may be used widely for the routine determination.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第6期729-732,共4页
Chinese Journal of Veterinary Science
基金
河南农业大学大学生"实践创新"项目(2007SJCX015)
国家自然科学基金资助项目(30671555)
河南省科技攻关重点资助项目(0423011600)
关键词
鸡志贺氏菌
凝集抗原
标准血清
研制
平板凝集试验
Shigella of chicken
agglutinating antigen
standard serum
development
plate agglutination test
