摘要
Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years. Expressed sequence tag （EST） analysis was carried out for this dinoflagellate in order to identify the functional genes involved in its biological processes. A cDNA library was constructed for P. donghaiense at exponential growth phase, and 565 usable sequencing reads were obtained from 700 clones selected randomly. Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons （EST groups）. Twenty-two EST groups were found to tag the genes involved in diverse biological processes including programmed cell death （PCD）. Two EST groups showed significant homologies with the encoding genes of cysteine protease （caspase） and proliferating cell nuclear antigen, respectively, two key proteins involved in PCD.
Prorocentrum donghaiense has caused large-scale red tides off the Chinese coast in recent years.Ex-pressed sequence tag(EST)analysis was carried out for this dinoflageilate in order to identify the func-tional genes involved in its biological processes.A cDNA library was constructed for P.donghaiense atexponential growth phase,and 565 usable sequencing reads were obtained from 700 clones selected ran-domly.Messenger RNA corresponding reads were clustered into 36 contigs and 272 singletons(ESTgroups).Twenty-two EST groups were found to tag the genes involved in diverse biological processes in-cluding programmed cell death(PCD).Two EST groups showed significant homologies with the encodinggenes of cysteine protease(caspase)and proliferating cell nuclear antigen,respectively,two key proteinsinvolved in PCD.
基金
Supported by the Key Laboratory of Mariculture of Chinese Education Ministry, Ocean University of China (No. 200405)
the Ministry of Science and Technology of China (No. 2005CCA02400,2001CB409700)
