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蒜氨酸酶灭活方法研究 被引量:8

Study on inactivated enzyme methods of alliinase in garlic
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摘要 对蒜氨酸酶灭活方法进行了研究以HPLC法测定蒜氨酸含量为指标,考察了微波、乙醇、微波-甲醇联合作用和热力灭酶4种方法的灭酶效果。结果表明,微波灭酶的最佳条件为:300kW、3 min;乙醇灭酶的最佳条件为:乙醇浓度90%(v/v),料液比1:4(w/v),处理30 min;微波-甲醇联合灭酶的最佳条件为:400 kW、1 min;热力灭酶的最佳条件为:90℃,40 min。 In this paper the effects of four kinds of inactivated enzyme methods, including microwave, alcohol, microwave- methanol and water heating, on the alliinase activity of garlic were studied. RP-HPLC was used to determine alliin in samples. The results showed that microwave method's optimum conditions is 300 kW, 3 min; alcohol method's optimum conditions is 90% (v/v), 1:4 (w/v) of material/water ratio, 30 min; microwavemethanol combined method's optimum conditions is 400 kW, 1 min; water heating method's optimum conditions is 90 ℃, 40 min.
出处 《食品科技》 CAS 北大核心 2009年第6期228-231,共4页 Food Science and Technology
基金 天津市科技计划项目课题(07ZCKFNC01800)
关键词 蒜氨酸酶 灭酶 高效液相色谱 Alliinase inactivated enzyme HPLC
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  • 1臧志清,周端美.大蒜药用和保健价值的综合开发[J].食品研究与开发,1999,20(1):25-28. 被引量:35
  • 2Stoll A, Seeback E. The enzymatic decomposition of alliinase and the properties of alliinase. Helv Chim Acta, 1949,32:197-205
  • 3Lancaster J E, Collin H A. Presence of alliinase in isolated vacuoles and alkyl cysteine sulphorides in the cytoplasm of bulbs of onion. Plant SciLett,1981,22:169-176
  • 4Whitetaker JR. Development of flavour, odor and plungency in lonion and garlic. Adv Food Res,1976,22:73-133

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