摘要
目的:研究麻黄种子发芽特性,为麻黄栽培生产提供依据。方法:种子发芽抑制物用流水冲洗法研究,种子活力用TTC法和红墨水法测定,生长物质对种子发芽的影响用琼脂培养基法,不同沙埋深度对种子发芽的影响用沙基培养基法研究。结果:麻黄干种子发芽率为44%,用蒸馏水浸泡处理12h种子萌发率为61%,用流水冲洗处理12h种子萌发率可达79.9%,而带苞片种子发芽率为0%。用40mg/LGA处理可明显促进麻黄种子发芽,2d就能达到最高萌发率94%。而用40mg/LIAA和40mg/L6-BA处理则明显延缓麻黄种子发芽,最高萌发率均延迟到第7d才出现,其发芽指数与对照间差异均达到显著水平(P<0.05)。在0～6cm沙埋深度范围内,随着沙埋深度的增加麻黄种子萌发率逐渐提高。而超过6cm,随沙埋深度的增大麻黄种子萌发率逐渐下降。随着沙埋深度增加,根长度呈抛物线形变化,而茎长度呈增加之势,根冠比递减。结论:麻黄苞片和种子均含种子萌发抑制物,充分的水洗、GA处理等能提高麻黄种子发芽率,加快种子发芽,同时适当深播可以提高麻黄种子发芽和出苗率。
Objective： To study the characteristics of the Ephedra sinica seed germination, to provide a basis for its cultivation. Methods：The seed germination inhibitive substances were studied by water washing method, the seed vigor was determined by TTC method and red ink method, the influence of growth substances to seed germination was studied by agar medium cultivation, and the influence of different sand burying depth on seed germination was studied by sand medium cultivation. Results： The seed germination rates of dry seeds, seeds as 12h soaking with distilled water, 12h washing by water and with ensheathe phyllary were 44%, 61%, 79.9% and 0% . Treated with 40 mg/L GA, the seed germination enhanced significantly as the maximum seed germination rate was 94% after 2 d. Treated with 40 mg/L IAA and 40 mg/L 6-BA, the seed germination delayed as the maximum seed germination rate appeared after 7 d, and the difference of seed germination index between treated and CK was significant （P 〈 0. 05）. In the sand burying depth 0 -6 cm, with the depth increasing, the seed germination rate gradually increased. But above 6 cm, when the depth increased, the seed germination rate gradually decreased. Moreover, as the sand burying depth increased, the root length changed in parabolashaped and the stem length increased, but the root top radio decreased. Conclusions：The phyllary and seed of Ephedra sinica contain some seed germination inhibitors, adequate water washing,dealing with GA and so on can improve the seed germination rate and speed up the seed germination. Appropriate deep sand burying can also improve seed germination and seedling emergence of Ephedra sinica.
出处
《中药材》
CAS
CSCD
北大核心
2009年第5期656-659,共4页
Journal of Chinese Medicinal Materials
关键词
草麻黄
种子萌发
沙埋
Ephedra sinica Stapf.
Seed germination
Sand burying
