摘要
目的观察Ⅰ型胶原（COLI）和Ⅲ型胶原（COLⅢ）在H2O2诱导的肝星状细胞（HSC）凋亡后的变化。方法用流式细胞术检测100nmol／L的H2O2诱导HSC不同程度的凋亡；应用半定量逆转录-聚合酶链反应（RT—PCR）检测细胞内COLⅠ、COLⅢmRNA的表达及变化。结果用100nmol／L H2O2诱导HSC凋亡（凋亡率分别5．86％、58．55％、71．98％），COLⅠ、COLⅢ在活化的HSC—T6细胞内均高表达，且伴随凋亡的增加，表达急剧下降，COLⅠ的变化更明显。结论诱导HSC凋亡可减少胶原基因的表达。
Objective To explore the changes of collagen （COL） Ⅰ and COLⅢ after apoptosis of HSC induced by H2O2. Methods Different apoptosis rate of HSC-T6 induced by H2O2 （ 100 nmol/L） was evaluated by flow cytometry. RT-PCR was used to detect the gene expression levels of COL Ⅰ and COL Ⅲ. Results Apoptosis of HSC was induced by 100 nmol/L H2O2 with the poptosis rate being 5.86%, 58.55 % , and 71.98 % , respectively. The COL Ⅰ and COL Ⅲ were highly expressed in activated HSC, and decreased sharply as apoptosis increased. The changes in gene expression of COL Ⅰ were much more obvious than those of COL Ⅲ. Conclusion Inducing apoptosis of HSC may decrease the gene expression of collagens.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第7期902-903,共2页
Chinese Journal of Experimental Surgery
关键词
肝星状细胞
脱噬作用
胶原
基因表达
Hepatic stellate cells
Apoptosis
Collogen
Gene expression
