摘要
目的探讨PTEN及P53的表达与胆管癌发生发展的关系,两者表达的相关性及其临床意义。方法应用免疫组织化学SP法对40例胆管癌标本,12例正常胆管组织和30例胆管癌旁组织中PTEN及P53蛋白表达情况进行研究。结果胆管癌中PTEN蛋白的阳性表达率为45.0%(18/40),明显低于正常胆管组织(100.0%,12/12)和癌旁组织90.0%(27/30),其表达与胆管癌的分化程度及有无转移有关而与部位无关。P53蛋白在胆管癌中的阳性表达率为40.0%(16/40),明显高于正常胆管组织0.0%(0/12)和癌旁组织3.3%(1/30)。两者的表达与胆管癌部位无关,但均与胆管癌的病理分级相关,PTEN与P53蛋白在胆管癌中表达具有相关性。结论PTEN蛋白在胆管癌中表达下降,并且其表达与胆管癌的分化程度及有无转移有关而与肿瘤部位无关。P53蛋白在胆管癌中表达升高,其表达与胆管癌的分化程度及有无转移有关而与肿瘤部位无关。胆管癌中PTEN与P53蛋白表达具有相关性。PTEN蛋白在胆管癌中低表达而P53蛋白在胆管癌中高表达,提示PTEN及P53在胆管癌的发生发展中起重要作用,P53突变可能下调PTEN的表达。
[Objective] To investigate the expression of phosphatase and tens in homolog deleted on chromosome 10 （PTEN） and P53 protein in cholangio carcinoma and evaluate the impossible relationship and clinical significance. [Methods] The expressions of PTEN and P53 in 40 cases of cholangio carcinoma, 12 cases of normal biliary duct and 30 cases of para-neoplastic tissue were detected by S-P immunohistochemistry method. [Results] The positive rate of PTEN protein expression was 45.0% （18/40） in cholangio carcinoma, which was significantly lower than that in normalbiliary duct 100.0% （12/12） and that in para-neoplastictissue 90.0%（27/30）, and which had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The positive rate of P53 protein expression was 40.0% （16/40） in cholangio carcinoma, which was significantly higher than that in no rmalbiliary duct 0.0% （0/12） and that in para-neoplastictissue 3.3% （1/30）, and it had no relationship with tumor locus, but was significantly correlated with the degree of differentiation and metastasis. The PTEN protein expression had correlation with the expression of P53. [ Conclusion] The expression of PTEN protein was significantly lower in cholangiocarcinoma and significantly correlated with the degree of differentiation and metastasis other than the locus of tumor. The expression of P53 protein was associated with the degree of differentiation and metasta- sis, but not with the tumor locus. Moreover, The PTEN protein expression had correlation with the P53 expression.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第11期1720-1723,共4页
China Journal of Modern Medicine
