摘要
目的：探讨β-七叶皂甙钠对严重烫伤早期大鼠突触可塑性的影响。方法：健康SD大鼠随机分为正常组、假烫伤组、烫伤组，烫伤后又设3、6、12、24、48h5个时相点，用透射电镜观察突触超微结构变化；免疫组织化学显色观察脑胶质原纤维酸性蛋白（GFAP）表达。结果：烫伤后神经元突触数量减少、星形胶质细胞线粒体肿胀、空泡化，GFAP表达增加。经β-七叶皂甙钠治疗后突触结构恢复，GFAP表达减少。结论：烫伤后大鼠皮质神经元突触结构遭破坏，β-七叶皂甙钠可能是通过保护神经元突触结构的基本完整，抑制GFAP表达，从而起到脑保护作用。
Objective： To explore the effects of β-aescinate sodium on synaptic plasticity in rat at the early stages of severe scald. Methods： Healthy SD rats were divided into three groups as following： normal control, sham scalded group and scald group, which was quantified at 3, 6, 12, 24, and 48 h following the thermal injury. The ultrastructural change of blood brain barrier was observed under a transmission electron microscope; The change of brain GFAP expression after severe scald was determined by immunohistochemical method. Results： Under the transmission electron microscope, the number of synapes and synaptic vesicle was decreased, and the mitochondrion of astrocytes of the cerebral cortex were swollen and vacuolated. And the expression of GFAP was significantly increased after burn. After treatmented by β-aescinate sodium, the structure of synapse was recoveried, expression of GFAP was decreased. Conclusion： Neuroprotection of β-aescinate sodium on severe scald rat might relate to its capability to maintain the integrity of synapes and inhibit the GFAP expression.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第3期367-370,共4页
Chinese Journal of Anatomy
