摘要
目的:探讨凋亡抑制蛋白Livin及线粒体促凋亡蛋白Smac/DIABLO和PTEN在胃癌发生发展分子机制中的调控作用及意义.方法:实时荧光定量PCR检测75例手术切除胃癌患者组织,20例癌旁组织及20例正常胃组织中LivinmRNA和Smac/DIABLOmRNA的表达,Westernblot结合免疫组织化学法检测两者与PTEN蛋白的表达及组织学定位.结果:正常胃组织及癌旁组织中均无LivinmRNA表达,胃癌组织中LivinmRNA相对表达量显著上调(6.374±4.759),其表达在低分化胃癌组及淋巴结转移组具有显著差异(χ2=9.60,5.51,P<0.01或0.05),与肿瘤大小,浸润程度及TNM分期等病理表现无关;Smac/DIABLOmRNA胃癌组织中的表达水平低于正常胃组织及癌旁组织,但差异无显著性(0.731±0.420vs1.104±0.276,1.061±0.737,均P>0.05),Smac/DIABLOmRNA的表达水平与胃癌各临床病理因素无关;其蛋白表达量与Smac/DIABLOmRNA的表达水平存在差异.Smac/DIABLO在肠型胃癌与弥漫型胃癌中的表达有显著差异(χ2=5.06,P<0.05).正常胃黏膜及胃癌组织中未检出PTEN蛋白表达.结论:Livin、Smac/DIABLO及PTEN的表达水平在不同阶段及病理类型的胃癌中存在差异.实时荧光定量PCR检测Livin及Smac/DIABLO的表达量,有可能为判断胃癌的发生,分化程度及预测化疗敏感性提供新的指标.
AIM： To investigate the regulating role and significance of protein Livin, mitochondrial apoptosis-promoting protein Smac/DIABLO and PTEN in carcinogenesis and progression mechanism. METHODS： Real-time RT PCR was used to examine the expression of Livin mRNA and Smac/DIABLO mRNA in 75 gastric carcino- ma specimens, 20 normal gastric tissues and 20 adjacent tissues. The expression and loca- tion of Livin, Smac/DIABLO and PTEN were detected using Western blot combined with immunohistochemistry （SP）.RESULTS： The expression of Livin mRNA was significantly up-regulated in gastric carci- noma specimens （6.374 ±4.759）, however, no expression was found in normal or adjacent tissues. There was a significance in expression between low differentiated carcinoma group and lymph node metastases group （x2 = 9.60, 5.51, P 〈 0.01 or 0.05）. The expression of Livin mRNA had no correlation with tumor size, invasion of nerve or TNM stage. The expression of Smac/DIABLO mRNA was lower in gastric cancer tissues than in normal gastric tissues and adjacent tissues, but there was no significant difference （0.731 ± 0.420 vs 1.104 ± 0.276, 1.061 ± 0.737, all P 〉 0.05）. The expression of Smac/DIABLO mRNA in gastric cancer tissues had no correlation with clinical pathological factors of gastric carcinoma. The expression of Smac/DIABLO held significant difference from intestinal-type gastric carcinoma to diffuse-type gastric carcinoma （x2 = 5.06, P 〈 0.05）. The expression of PTEN was not determined in gastric carcinoma tissues and normal tissues. CONCLUSION： There is a diversity of expression of Livin, Smac/DIABLO and PTEN in dif- ferent stages and pathological types of gastric carcinoma. Real-time RT PCR and the expression of Livin and Smac/DIABLO could be beneficial to diagnosis of gastric carcinogenesis, severity of differentiation, and chemotherapy sensitivity.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第13期1303-1311,共9页
World Chinese Journal of Digestology
基金
辽宁省自然科学基金资助项目
No.2008225008
沈阳市自然科学基金资助项目
No.080411~~
