摘要
本研究采用致细胞病变(cytopathic,CP)型牛病毒性腹泻病毒(bovine viral diarrhea virus,BVDV)标准毒和非致细胞病变(noncytopathic,NCP)型新疆优势毒株免疫产蛋鸡,用改良PEG法提取卵黄抗体(IgY),并对提取的IgY采用SDS-PAGE检测纯度,间接ELISA检测免疫后每隔7 d的抗体效价,并测定所得抗体对NCP型BVDV的中和效价。结果表明,用该法提取的IgY纯度较高;间接ELISA结果证明,经过4次免疫后,抗CP型BVDV的效价达到1∶32000,抗NCP型BVDV的效价达到1∶40000,3个月后再次检测,卵黄抗体效价未见明显下降。最后一次免疫14 d的抗体对NCP型BVDV的中和效价达到1×10-3。
Layer hens were immunized with cytopathic （CP） and noncytopathic （NCP） bovine viral diarrhea virus （BVDV）, the egg yolk was successfully treated with modified PEG6000 method. The purity of IgY was detected with SDS- PAGE and the IgY titer was assayed by indirected ELISA after 7 days, and the neutralizing antibody titer to NCP BVDV with immunoperoxidase monolayer assay （IPMA）. The results suggested that higher purity of lgY was observed with the use of modified PEG6000. We can detect anti-BVDV IgY in the first 14 days. After being immunized with 4 doses of the conjugate, the titer of IgY against CP BVDV was 1 ： 32000, against NCP BVDV was 1 ： 40000 and kept the conditions after 3 months. Neutralizing antibody titer to NCP BVDV was 1×10^-3.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第6期124-128,共5页
China Animal Husbandry & Veterinary Medicine
基金
兵团博士资金项目
兵团科技攻关项目(2007Z-X02)
