摘要
通过添加血红素,部分乳酸菌种(株)的细胞在有氧条件下可进行有氧呼吸代谢,细胞具有完整的呼吸链;遗传学证据表明,细胞色素氧化酶cydA基因可以在对数生长末期获得表达,代谢调控蛋白CcpA作为有氧代谢的"开关"进行代谢调控,此时乳酸菌细胞具有耐氧和耐酸性能力。相对于发酵条件而言,有氧代谢可促使乳酸菌细胞获得较高的生长量,培养介质的pH值维持在较高水平,使细胞具有较强的抗逆效果和生存能力。这意味着通过控制性地改变乳酸菌的培养条件,或许能改善乳酸菌细胞在有氧条件下的增殖潜力和生存能力,拓宽其在食品发酵工业中的应用范围。
Several strains (species) of lactic acid bacteia (LAB) such as Lactococcus lactis can undergo respiration metabolism and thrive in the presence of both oxygen and a heine source. Genetic evidence suggests that cydA genes encoding cytochrome d oxidase are expressed at the end of log phase, and aerobic respiration is regulated by the catabolite control protein A (CcpA) acting as "a switch" in LAB strains, therefore the strains present some oxygen and acid resistance. Compared to fermentation, aerobic resptration can prompt effectively the achievement of high biomass, the maintenance of high pH value of culture medium and the increases of adverse resistance and survival ability. It means that the controlled changes in cultivation conditions may increase the proliferation potential and survival ability of LAB, which can widen their application scope in food fermentation industry.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第13期262-267,共6页
Food Science
基金
国家"863"计划项目(2006AA10Z344
2008AA10Z335)
关键词
乳酸菌
有氧增殖
呼吸代谢
遗传学特征
lactic acid bacteria
aerobic proliferation
respiration metabolism
genetic characteristics