摘要
目的观察砷对大鼠脑细胞能量代谢和超微结构影响,探讨砷的中枢毒性作用和机制。方法SD成年大鼠,随机分为对照、低砷、中砷、高砷组和繁殖仔代组,染砷组自由饮三氧化二砷(AS2O3)水。6和16周后,测定成鼠和仔鼠脑细胞线粒体三磷酸腺苷(ATP)合成量并观察脑细胞超微结构变化。结果成鼠砷暴露6和16周后,脑细胞线粒体ATP合成量〔低砷6周(222.90±3.85)nmol/(mg.pr);16周(166.12±4.40)nmol/mg.pr〕比对照组〔(238.57±1.31)nmol/(mg.pr)〕明显减少,并与暴露剂量和暴露时间呈正相关;高砷繁殖组孕产的仔鼠出生后继续高砷喂养16周,ATP合成量〔(28.66±2.32)nmol/(mg.pr)〕比成鼠16周高砷组〔(54.25±4.92)nmol/(mg.pr)〕降低更明显。电镜观察染砷6周组脑细胞粗面内质网扩张,线粒体肿胀数目减少;16周中、高砷组还出现核肿胀和细胞器减少;16周仔代组核内结构疏松,线粒体等细胞器数目明显减少,胞质部分区域有空泡现象。结论慢性砷暴露对大鼠脑细胞超微结构和能量代谢功能有明显损伤作用,仔鼠损伤程度比成鼠更严重。
Objective To investigate the toxic effects of arsenic on brain cellular energy metabolism and ultrastructure in rats. Methods Healthy adult SD rats were randomly divided into five groups（ control,low-, moderate- and high-dose arsenic and offspring-producing groups） and the rats in experimental groups were exposed to drinking water containing AS2O3. After 6 and 16 weeks,ATP contents of brain cellular mitochondria were determinined in all groups and cellular ultrastructure changes were observed. Results After arsenic exposure, ATP contents （222. 90 ± 3. 85 nmol/mg, pr for 6 weeks low arsenic exposure, 166. 12 ± 4. 40 for 16 weeks low arsenic exposure）in brain cellular mitochondria decreased compared to that of the control group（238.57 ± 1.31 nmol/mg-pr）and positively correlated with the exposure dose and time. The contents of ATP in the offspring group（28. 66 ± 2. 32 nmol/mg-pr） was much lower than that of adult rats（54. 25 ±4. 92 nmol/mg·pr） after exposed to high concentration of arsenic for 16 weeks. The ultrastructures of brain cells showed enlarged rough endoplasmic reticulum and swellen mitochondria. In moderate and high arsenic groups, nuclear swelling and decreased number of organelles were found after exposed to arsenic for 16 weeks. In the offspring-producing group, the intranuclear structures became loose and the number of mitochondria organelles decreased obviously, with porous cytoplasm. Conclusion Arsenicalism causes evident damage of cellular energy metabolism and ultrastructure in rat brain. And the fetus rats are severely affected than the adults rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2009年第7期878-879,共2页
Chinese Journal of Public Health
基金
贵州省科技厅科技攻关项目(1123)
关键词
砷
脑细胞
三磷酸腺苷
超微结构
arsenic
brain cell
adenosine triphosphate
ultrastructure
