摘要
目的分离人原发性食管组织中新的相关基因，揭示食管癌的易感性与癌变原理。方法用高效、灵敏的ｍＲＮＡ差异显示技术，以２例正常食管上皮、１例癌旁上皮、２例高癌家族的食管癌组织互为对照，通过对其基因表达的比较，找出差异条带，进行ＲＴＰＣＲ鉴定和ＤＮＡ序列分析。结果（１）在实验中，分离、鉴定了１８个差异片段，其中包括正常组织表达而肿瘤不表达的正常食管基因（ｎｏｒｍａｌｅｓｏｐｈａｇｅａｌｇｅｎｅ，ＮＥＧ）１３个，肿瘤表达而正常组织不表达的突变食管基因（ｍｕｔａｔｅｄｅｓｏｐｈａｇｅａｌｇｅｎｅ，ＭＥＧ）５个。（２）４个ＮＥＧ片段在ＧｅｎＢａｎｋ数据库中没有发现同源的已知基因或片段，将其分别命名为食管癌相关基因１～４（ｅｓｏｐｈａｇｅａｌｃａｎｃｅｒｒｅｌａｔｅｄｇｅｎｅ，ＥＣＲＧ１～４）。（３）其他１４个ｃＤＮＡ片段分别与ＧｅｎＢａｎｋ中的１２个已知基因或片段同源，这些基因在食管癌中的作用还有待于进一步研究。（４）通过ＲＴＰＣＲ鉴定，已初步证明上述４个ＥＣＲＧ片段在正常食管上皮组织和食管癌中的表达有差异。（５）通过对７个人正常组织的ｃＤＮＡ文库检测表明，这４个ＥＣＲＧ片段在胎脑、成人脑、肝、肾、睾丸、骨髓及骨胳肌?
Objective To search new genes related to human esophageal cancer(EC) for revealing carcinogenesis mechanism and genetic susceptibility of EC. Methods Three normal esophageal epithelia(including 1 tumor adjacent tissue) and two primary squamous cell carcinomas collected from high incidence family in Lin xian county were studied using technique of mRNA differential display. The differential fragments were sequenced and identified by RT PCR assay. Results (1)Eighteen differential fragments were isolated and identified, 13 of which were expressed in normal esophageal epithelia but not in EC(assigned as normal esophageal gene, NEG), while 5 were expressed in EC but not in normal esophageal epithelia(assigned as mutated esophageal gene, MEG). (2)Four NEG fragments were not homologous to the known sequences in the public database of GenBank (of NLM in USA). These 4 fragments were assigned as esophageal cancer related gene (ECRG) 1 to 4. (3)The remaining 14 fragments were homologues to 12 known genes or gene fragment. Their role in EC remains unclear. (4) Using RT PCR technique, the expression of ECRG between normal epithelia and EC was significantly different. (5) All 4 ECRG genes were expressed in cDNA libraries derived from normal fetal brain, adult brain, liver, kidney, testis, bone marrow and skeletal muscle. (6) In the 20 cancerous and tumor adjacent tissues obtained from the liver, lung, breast, colo rectum and endometria, ECRG1 and ECRG2 was not detected by RT PCR, while ECRG3 was highly expressed. For the ECRG4 the expression was much stronger in tumor adjacent tissues than in cancerous tissues. Conclusion ECRG 1 and ECRG2 may contribute to the causation and progression of the EC in Lin xian, and may be candidates of tumor suppressor genes.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1998年第4期254-257,共4页
Chinese Journal of Oncology
基金
国家八五科技攻关基金
国家教委基金
关键词
食管肿瘤
基因表达
CDNA
MRNA
聚合酶链反应
Esophageal neoplasms Gene expression RNA, messenger DNA, complementary Polymerase chain reaction
