摘要
目前,血吸虫病仍然是许多国家和地区比较严重的公共卫生问题,寻找合适有效的防治方法是控制以至消灭血吸虫病工作的重点和难点。RNA干扰技术以其高效、特异、快捷地阻断特定基因的表达的优势,近年来已成为基因功能研究、基因防治和寻找药物靶点的潜在的强有力工具。本文介绍了RNA干扰技术在血吸虫学研究中的应用,包括在血吸虫生活史的不同生长发育阶段、借助不同的载体等方法实施RNA干扰进行血吸虫相关基因的功能研究,以及对其抗感染和抗病治疗的效果进行评价,以助于血吸虫生长发育及致病的一些重要基因的功能研究,为探索防治血吸虫病的新方法提供一些思路。
To date, the schistosomiasis remains to be an important public health problem in many regions and countries, and it is primary focus to determine an effective way to control this disease. Because of its high efficiency, specificity, and speed to block a particular gene expression, the RNA interference technology has become a potential powerful tool for the research of gene {unction, gene prevention and treatment as well as drug targets. This article introduces the applications of RNA interference in schistosome research in the past decades, including studies of gene functions during different periods of schistosome life cycles and through different RNAi methods, the evaluation on the effect of anti-infection and nosotropic treatment. This is essential in order to fully understand some special genes which are important to growth and development for schistosome and to determine a new platform for anti-schistosomiasis.
出处
《中国病原生物学杂志》
CSCD
2009年第6期460-463,共4页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.30872202
No.30571632)
湖北省卫生厅血防重点项目(No.XF2008-5)
关键词
RNA干扰
血吸虫
研究
应用
综述
RNA interference
schistosome
research
application
review
