摘要
目的:建立一种更方便、快速、安全的检验血清HBsAg的手段。方法:采用胶体金免疫层析分析方法。结果:检测临床及血库血清样品189例,与酶联免疫吸附试验(ELISA)对照比较,二者检测符合率为98.9%,检测灵敏度可达5μg/L以下。结论:该方法检测灵敏度、特异性等指标已接近ELISA水平,并且检测操作更方便、快速,有希望发展成为一种新的临床检测方法。
Objective:To develop a methed to detect serum HBsAg which was more simple.rapid and safe.Metheds:Colloidal gold immunochromatography was selected selected. Results: In detecting 189 serum sapmles fromclinic and blood bank, this method had a coincidence rate of 98. 9% with ELISA. Detection sensitivity couldreach 5μg/L or even lower. Conclusion: The detection sensitivity and sepecificity by this method approach thoseby ELISA and it could be developed to be a new clinical detecting method because of its simpleness and rapidness in operation.
出处
《白求恩医科大学学报》
CSCD
1998年第4期430-431,共2页
Journal of Norman Bethune University of Medical Science