摘要
目的:探讨不同睡眠剥夺时间对大鼠海马和前额皮层神经颗粒素(Ng)、蛋白激酶C(PKC)、Ca2+-CaM依赖性蛋白激酶Ⅱ(CaMKⅡ) mRNA表达的影响。方法:雄性Wistar大鼠随机分为3组,即24h实验组、48h实验组和72h实验组,每组再分为睡眠剥夺组(SD组)和对照组(C组)。利用睡眠剥夺箱建立大鼠SD模型,Trizol一步法提取海马和前额皮层总RNA,用SYBR AgreenⅠ荧光定量RT-PCR测定Ng、PKC和CaMKⅡ mRNA水平。结果:(1)大鼠海马Ng mRNA水平在睡眠剥夺24h、48h和72h后,较对照组均显著降低(P<0.05),PKC和CaMKⅡ mRNA水平在睡眠剥夺48h和72h后显著降低(P<0.05);(2)在睡眠剥夺24h和48h后,大鼠前额皮层Ng、PKC和CaMKⅡ mRNA水平较对照组有降低趋势,在睡眠剥夺72h后均显著降低(P<0.05)。结论:睡眠剥夺可以使大鼠海马和前额皮层Ng、PKC和CaMKⅡ mRNA表达水平降低,且随着睡眠剥夺时间的延长,降低更为明显,这可能是睡眠剥夺损害认知功能的原因之一。
AIM： To study the effects of different duration of sleep deprivation （SD） on neurogranin （Ng）, protein kinase C （PKC） and Ca^2 -calmodulin dependent protein kinase Ⅱ （CaMK Ⅱ） mRNA expressions in hippocampus and forebrain of rats. METHODS： Male Wistar rats were divided into three groups ： 24 h, 48 h and 72 h experimental groups. Each group was divided into sleep deprivation group （SD group） and control group （C group）. SD model was es- tablished by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng, PKC and CaMK Ⅱ mRNA expressions were detected by SYBRA green Ⅰ RT - PCR. RESULTS： （ 1 ） The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h, 48 h and 72 h SD （P 〈 0. 05）. The expressions of PKC and CaMK Ⅱ mRNA in SD group were significantly lower than those in C group after 48 h and 72 h SD （ P 〈 0. 05 ）. （ 2 ） The expressions of Ng, PKC and CaMK Ⅱ mRNA in forebrain of SD group were lower than those in C group after 24 h and 48 h SD, and significantly lower than those in C group only after 72 h SD （ P 〈 0. 05 ）. CONCLUSION ： The expressions of Ng, PKC and CaMK Ⅱ mRNA are down regulated by SD, which might be relevant to the impairment of cognitive function in rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第7期1355-1359,共5页
Chinese Journal of Pathophysiology
