摘要
目的对临床分离大肠埃希菌株携带的Ι类整合子及相关耐药基因进行筛选和分析,探讨Ι类整合子在大肠埃希菌耐药中的作用。方法对43株大肠埃希菌临床分离株做药敏试验;采用PCR扩增、DNA测序、DNA序列比对的方法对其携带的Ι类整合子相关耐药基因进行分析。结果43株大肠埃希菌分离株对10种抗菌药物的耐药率依次为亚胺培南4.7%、阿米卡星18.6%、头孢他啶、27.9%、头孢吡肟37.2%、头孢呋辛55.8%、复方磺胺甲噁唑58.1%、妥布霉素74.4%、庆大霉素79.1%、头孢噻肟81.4%和哌拉西林83.7%。在43株大肠埃希菌分离株中有25株含有Ι类整合子,其中18株携带整合子相关耐药基因,如介导对磺胺类和氨基糖苷类药物的耐药基因等;某些菌株携带的整合子相关耐药基因相同。结论Ι类整合子在大肠埃希菌中广泛存在,整合子相关耐药基因在该菌耐药性的形成和播散中发挥作用。
Objective To study distribution of the class 1 integron in clinical Escherichia coli isolates and explore the relationship between integron and their drug resistance. Methods The class 1 integron and drug resistance gene cassettes integrated by class 1 integron among the E. coli isolates were analyzed by PCR screening, DNA sequencing and sequence analysis, and the antibiotic sensitivity tests were performed. Results Twenty five of 43 E. coli isolates were observed in class 1 integron, 18 of which carried the integrated drug resistance gene. Susceptibility test showed their resistance as follows 4.7% of imipenem, 18.6% of amikacin,27.9% of ceftazidime ,37.2% of cefepime, 55.8% of cefuroxime,58.1% of sulfamethoxazole,74.4% of tobramycin,79.9% of gentamicirt,81.4% of cefotaxime and 83.7% of piperacillin. Conclusion Drug resistance genes integrated by class 1 integron were widespread in E. coli isolates.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2009年第7期434-436,共3页
Chinese Journal of Antibiotics
基金
广东省自然科学基金项目(8452402301001324)
广东医学院科学基金(XB0620)
湛江市科技计划项目
东莞市科技计划项目
