摘要
目的利用兔同种异体软骨脱细胞基质支架和脂肪干细胞体外构建组织工程软骨,探讨其修复关节软骨损伤的可行性。方法将新西兰大白兔的脂肪干细胞与软骨脱细胞基质支架复合,于软骨细胞方向诱导培养基中培养两周,构建组织工程软骨。兔24只随机分为A、B、C 3组,A组关节软骨缺损处置入经诱导的脂肪源干细胞复合软骨基质支架,B组缺损处只置入软骨基质支架,C组软骨缺损处不做任何处理。分别于术后第12周处死动物,修复处行大体、组织学、Ⅱ型胶原免疫组化染色和透射电镜检测。结果A组软骨缺损处被类软骨组织填充,修复区表面光滑;Ⅱ型胶原免疫组化染色和甲苯胺蓝染色阳性;电镜下可见软骨陷窝内有细胞结构存在,且有大量均匀颗粒状细胞分泌基质成分存在,细胞周围大量胶原纤维。B组软骨缺损处为纤维组织状物填充,C组软骨缺损处无修复组织填充。结论脂肪干细胞与软骨脱细胞基质复合并向软骨诱导后可良好地修复关节软骨缺损,具有替代正常软骨的潜力。
Objective To evaluate the healing behavior of rabbits articular cartilage defects repaired with tissue engineered cartilage constructed by acellular articular cartilage matrices（AACMs）seeded with adipose derived stern cells （ADSCs）.Methods ADSCs with rabbit-induced were seeded in AACMs with rabbit-developed and cultured in chondrogenie differentiation medium for two weeks in order to construct tissue engineered cartilage. 24 New Zealand white rabbits were divided into A, B and C groups randomly. Then engineered cartilage was transplanted into cartilage defect position of rabbits in the group A. AACMs was for the group B and nothing for the group C. The rabbits were killed in the 12th week. Restored tissue was evaluated using naked eyes, histology, immunohistochemistry and transmission electron microscope （TEM）. Results In the group A, articular cartilage defects of the rabbits were filled with chondrocyte-like tissue which cells were positive of Alcian blue staining and type IT -collagen either in expression. The chondrocytes can be seen in the recesses and there were plenty of the matrix particles and collagen surrounding the ceils with TEM scanning. In the group B, the defect was filled with fibrous tissue. No tissue was found in the group A. Conclusions Tissue engineered cartilage using AACMs seeded with ADSCs cultured in chondrogenic differentiation medium can be well repair of articular cartilage defects in rabbits. It has the potential to substitute for the normal cartilage.
出处
《中国骨质疏松杂志》
CAS
CSCD
2009年第7期501-505,共5页
Chinese Journal of Osteoporosis
基金
贵州省科技厅和珠海市医学重点专科建设基金资助项目:黔科合NY字[2006]3036珠卫[2008]80
关键词
脂肪干细胞
软骨脱细胞基质
软骨
组织工程
Adipose-drived stem cell
Acellular cartilaginous matrix
Cartilage
Tissue engineering
