摘要
采用9对微卫星引物分析了凡纳滨对虾4个选育群体(Molokai、OI、SIS和Kona Bay)的遗传多样性。结果表明:9对微卫星引物共检测到32个等位基因,每个位点等位基因数2～7个,平均3.555 6个,平均有效等位基因数2.472 7;各位点的观测杂合度(Ho)0.026 0～0.623 4,期望杂合度(He)0.263 1～0.785 5;各群体平均观测杂合度从小到大依次为SIS(0.383 3)<Kona Bay(0.405 2)<Molokai(0.427 8)<OI(0.444 4)。群体间基因分化系数(Gst)为0.153 8,各群体之间存在较大遗传分化。群体间遗传相似性系数、遗传距离及UPGMA聚类分析表明,OI与Kona Bay群体亲缘关系最近,其次是Molokai与Kona Bay群体,而SIS与Molokai群体亲缘关系最远。
Genetic diversity of 4 selected stocks （Molokai, OI, SIS and Kona Bay） of Litopenaeus vanname was assessed by 9 microsatellite markers. The results showed that the number of total alleles were 32. The numbers of allele of every locus was ranged from 2 to7. The mean allele number （Na） was 3.5556, Heterozygosity value （Ho） observed was 3.5556. Observed heterozygosity value （Ho） was 0.0260 to 0.6234 and expected heterozygosity value （He） was 0.2631 to 0.7855. The order of mean Ho values was SIS （0.3833）, Kona Bay （0.4052）, Molokai （0.4278） and OI（0.4444）. The coefficient of gene differentiation between the stocks （Gst） was 0.1583, there were major genetic differentiation among these 4 selected populations. Based on the results of genetic similarity, the genetic distance and UPGMA dendrogram was constructed. And we found that the relationship of population of OI and Kona Bay was the closest, and Molokai and Kona Bay popilation were followed. SIS and Molokai population have the most distant relationship. The results may provide a theoretical basis for conservation, exploitation and breeding ofLitopenaeus vannamei.
出处
《广东海洋大学学报》
CAS
2009年第4期5-9,共5页
Journal of Guangdong Ocean University
基金
广东省科技厅科技创新项目2008A023700011
关键词
凡纳滨对虾
选育群体
遗传多样性
微卫星
Litopenaeus vannamei
selected stocks
genetic diversity
microsatelite
