摘要
利用RT-PCR、RACE等技术,从大黄鱼胃组织中克隆得出Ghrelin基因的cDNA(649 bp),其中包含5’非翻译区(5’UTR),起始密码子,信号肽、成熟肽、C-端肽序列的编码区,终止密码子,3’非翻译区(3’UTR)和相对较少见的多核苷酸化信号(ATTAAA).获得的cDNA编码108个氨基酸,与已报道的硬骨鱼类Grelin mRNA比对显示,相似性(Simi-larity)和一致性(Identity)最高(黑鲷Acanthopagrus schlegelii)可达81%和73%,推测的成熟肽包含硬骨鱼Ghrelin的相同活性中心和修饰位点,证明是鱼类Ghrelin同源基因.
By using RT-PCR and smart-RACE, a Ghrelin eDNA fragment was isolated from stomach of large yellow croaker, Pseudosciaena crocea. The amplified eDNA was totally 649 bp in length including 5＇ UTR, coding region, 3＇ UTR and a non-canonical polyadenylation signal （ATTAAA）. and encoding 108 amino acids which composed signal peptide, mature peptide and C-terminal peptide. The isolated eDNA showed 81%similarity and 73% identity after BLAST with eDNA from black porgy,Acanthopagru, schlegelii. The mature peptide composed 22 amino acids, with a molecular weight of 2 310. 57 u, showed high similarity with other teleost fishes, and strongly indicated that Ghrelin eDNA from large yellow croaker was a homologous gene of teleost ghrelin.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期613-616,共4页
Journal of Xiamen University：Natural Science
基金
国家863计划(2006AA10A405)项目资助
