摘要
背景:壳聚糖是甲壳素的脱乙酰产物,这种碱性氨基多糖具有天然抗菌活性,且抗菌广谱,但将其纳米化后抗幽门螺杆菌作用的报道较少。目的:制备壳聚糖纳米粒并评价其体外抗幽门螺杆菌的疗效。设计、时间及地点:体外对比观察实验,于2008-03/10在中南大学湘雅医院卫生部纳米生物技术重点实验室完成。材料:采用聚合物分散法用脱乙酰度分别为88.5%和95%的2种壳聚糖粉末制备壳聚糖纳米粒。方法:取0.5mL含幽门螺杆菌(NCTC11639和NCTC11637)5×108CFU/mL的布氏肉汤菌液均匀涂布于培养基上待干燥后,采用打孔法检验不同质量浓度(5,10,15,20g/L),不同pH值(pH=6,5,4)和不同脱乙酰度(88.5%和95%)壳聚糖纳米粒溶液的抑菌效果。主要观察指标:抑菌环直径。抑菌环直径越大,说明该菌对此药敏感性越大,抗菌效果越好;反之越小,抗菌效果越差;若无抑菌环,则说明该菌对此药具有耐药性。结果:pH值在4～6时,壳聚糖纳米粒的抑菌作用与pH值呈负相关(P<0.01),且在pH值为4时的抑菌效果最强。2种不同脱乙酰度壳聚糖对幽门螺杆菌的抑菌作用差异有显著性意义(P<0.05),壳聚糖纳米粒(脱乙酰度95%)的抑菌效果优于壳聚糖纳米粒(脱乙酰度88.5%),在质量浓度处于5～20g/L时抗幽门螺杆菌的作用差异无显著性意义(P>0.05)。结论:壳聚糖纳米粒提高了在体外对幽门螺杆菌的抑菌作用,且此作用在酸性条件下明显增强。用脱乙酰度为95%的壳聚糖粉末制备的壳聚糖纳米粒具有更好的抑幽门螺杆菌的作用。
BACKGROUND： Chitosan （CS）, an amino polysaccharide, is deacetylated from chitin, which has natural substances with antibiotic activity and wide antibacterial spectra. However, the effect of nano structured chitosan on anti-Helicobacter pylori （HP） is poorly understood. OBJECTIVE： To formulate and systematically evaluate anti-Hp efficacy of CS nanoparticles （NPs） in vitro. DESIGN, TIME AND SETTING： The in vitro control experiment was performed at the National Key Laboratory of Nanobiological Technology, Xiangya Hospital, Central South University from March to October 2008. MATERIALS： CS NPs were prepared by the polymerid dispersion method with 88.5% and 95% deacetylation. METHODS： Totally 0.5 mL Brucella Broth containing NCTC11639 and NCTC11637 5×10^8 CFU/mL were spread on culture medium. The anti-Hp effect of different concentration （5, 10, 15, 20 g/L） in vitro, different PH value （pH=6, 5, 4） and different deacetylation degree （88.5% and 95%） of CS NPs solution was detected with punch method. MAIN OUTCOME MEASURES： The diameter of inhibition zone, which demonstrated the antibacterial effect. No antimicrobial ring illustrates that the bacterium has drug resistance. RESULTS： At pH value 4-6, the anti-Hp effect of CS NPs solution had a negative correlation with pH value （P 〈 0.01）, and the optimal pH value was 4. There were significant differences in anti-Hp effect between 88.5% deacelytation degree CS NPs and 95% deacelytation degree CS NPs （P 〈 0.05）, which was superior in 95% deacelytation degree CS NPs, however, the anti-Hp effect of showed no difference within 5 20 g/L （P 〉 0.05）. CONCLUSION： CS NPs improves the anti-Hp efficacy of CS in vitro and has the potential to kill Hp directly, and the action is strengthened in acid condition. 95% deacelytation degree CS can enhance the bacteriostatic activity of the chitosan nanoparticles.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第29期5695-5698,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
