摘要
漆酶是一种广泛分布在真菌中的酶,其底物有非专一性,在木质素的降解和环境保护方面有重要作用。为测定和估计平菇菌丝液体发酵期间的漆酶产生量,使用紫外-可见分光光度计对菌液进行了全波长扫描,结合常规化学测定方法和平板固体培养基培养时的漆酶圈直径来进行研究,探明粗酶液全波长光谱特性与漆酶产生量的关系。结果发现,发酵粗酶液全波长扫描图谱在300nm处的波峰数目与漆酶的产量呈现相关关系;300nm处吸光值大于1.5的波长范围逐渐增大,11天的培养期间其波长范围从5nm变化到80nm与漆酶的产量呈正相关关系;在320～400nm出现有规律的光吸收,且在349,365,388nm处各出现光吸收峰,其中在365nm处出现最大吸收,且吸光值均与漆酶的产量呈正相关关系。据此可以应用于粗酶液中漆酶含量的估计或测定。与常规化学分析相比,全波长扫描方法有测定速度快、简单、节省溶剂、无化学污染等优点,为实际工业生产中的漆酶产量估计和测定提供了新方法。
Laccase, widely distributed in fungi lacking high substrate specificity, plays an important role in lignin degradation in nature and environmental protection. In order to determine or estimate the laccase production during the fermentation of liquid media, the authors studied the full-length wave scan on the rough fermentation liquid of the Pleurotus ostreatus, which produces laccase high. Combined with the normal chemical method and diameter of the laccase and mycelium stain, which grew on the PDA （potato dextrose agar） plate with guaiacol added, we could get the exact information of laccase production. The result showed that the laccase activity increased in a rapid way in the first 5 days during the fermentation process, remained almost at the same level in the following 4 days, then increased rapidly until the 11 day, which was 148. 7 U . L^-1 , increased 17. 9 times. The diameter of laccase and mycelium stain increased with the culture time. The number of the wave peaks around 300 nm had a positive correlation with the laccase production; the peak width of OD over 1.5 around 300 nm had a positive correlation with the laccase production, which ranges from 5 nm on the first day to 80 nm on the 11th day. The light absorption line between the wavelengths 300 and 400 nm had a positive correlation with the laccase production with peaks at 349, 365 and 388 nm, and at 365 the peak gets its highest. Using these parameters, the authors could get the general production of the laccase production of liquid fermentation. Compared with the normal chemical method, the full-length wave scan method is much easier, cheap and simple. Furthermore, there are no special chemical substances used. It is really a new method for the evaluation and determination of laccase.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2009年第8期2157-2160,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(30671471
30571291)资助
关键词
全波长扫描
光谱特性
漆酶
产生量估计
Full-wavelength scan
Spectrum character
Laccase
Estimate of the production
