摘要
目的探讨氯胺酮预处理对兔肺缺血再灌注损伤中微血管内皮细胞、核因子-κβ和肿瘤坏死因子-α的影响。方法采用改良Sekido法制备兔肺缺血再灌注损伤(Lung Ischemia-Reperfusion Injury LIRI)模型,随机分为假手术组(C组)、缺血再灌注损伤组(IR组)、氯胺酮预处理(KET组)。观察氯胺酮对兔肺缺血再灌注损伤的保护作用及其机制。结果KET组与IR组相应时间点相比核因子-kappaβ(Nuclear Factor-kappa BNF-κβ)蛋白表达降低显著;肿瘤坏死因子-α(Tumor Necrosis Fector-α TNF-α)、湿干重比(W/D)和肺泡损伤指数(the index of quantitative assessment of histologic lung injuryIQA)有不同程度降低。光镜观察到KET组较IR组肺间质水肿程度轻,红细胞渗出不明显。电镜下KET组偶见线粒体空泡化,细胞核完整清晰。NF-κβ蛋白表达与血浆TNF-α含量成显著正相关。结论氯胺酮可能通过下调核转录因子活性进而抑制TNF-α等炎症介质的表达,保护VEC,介导了对缺血再灌注肺组织的保护作用。
Objective To explore the effects of katamine preconditioning on the microvascular endothelial cell. nuclear factor-kappa β （NF- κβ） and tumor necrosis factor-α （TNF-α） in rabbits with lung ischemia reperfusion injury. Methods The healthy mature rabbits were randomly divided into 3 groups, the sham operation group （C group）, the ischemia-reperfusion injury group （IR group） and the ketamine preconditioning group （KET group） after establishing model of lung ischemia reperfusion injury （LIRI） with the modified Sekido method in order to observe the pretective effects and mechanism of katamine. Results Comparing whh the IR group, protein expression of NF-κβ significantly decreased in KET group. All of TNF-n, wet to dry weight ratio （W/D） and the index of quantitative assessment of histological lung injury （IQA） decreased at different degree. Comparing with IR group, the degree of pulmonary interstitial edema was mild in KET group, and the effusion of red blood cells was not obvious under light microscope. Mitochondrial vacuolization in capillary endothelial cell with clear and complete cell nucleus was observed Occasionally under electron microscopy. There was a significant positive correlation between protein expression of NF-κβ and the plasma concentration of TNF-α. Conclusion Ketamine may restrain the expression of inflammatory mediators such as TNF-α by down-regulating the activity of nuclear transcription factors, and play a role in protecting the issues in lung ischemia reperfusion injury.
出处
《浙江预防医学》
2009年第8期10-14,共5页
Zhejiang Journal of Preventive Medicine
关键词
氯胺酮
肺
再灌注损伤
Ketamine
Lung
Reperfusion injury
