摘要
为获得一组常温条件下加速木质纤维素腐解的菌群,利用限制性培养法,筛选到一组在室温(28℃)条件下,5 d可分解天然稻秆总重39.6%的菌群.仅用质谱仪的一个CP-Chirasil-Dex CB毛细管柱就能够检测到丙酸、乙醇、异丙酸、4-氨基-1-丁醇、丁酸、硅烷-二乙基、乳酸、乙二醇、邻苯二甲酸二乙酯、甘油等10种以上有机物.定量分析挥发性产物,发现其种类和浓度随时间变化很大.用变性梯度凝胶电泳(DGGE)检测菌群的动态变化,发现在培养不同时期菌种组成差异很大,通过对各条带近缘种网上比对结果可见,该菌群具有丰富的菌种组成多样性,菌群内微生物分别归属Clostridiumsp.、Brevibacillussp.、Rhizobiumsp.、Bacteriumsp.等4个属.
A normal temperature lignocellulose-degrading microflora has been constructed by our laboratory. We researched the degradation activity and compose of the community in 2822 fermentation condition. The results showed that the microbial community could degrade 39.6% of rice stew gross weight within five days. The volatile products were detected using CP-Chirasil-Dex CB capillary column by GC-MS, propionic acid, ethanol, isopropyl alcohol, 4- amino- 1 - butanol, butanoic acid, diethoxydimethyl- silane, lactic acid, ethanol, 2,2＇- oxybis-, diethyl phthalate and glycerin, more than 10 kinds of volatile products were detected. The state volatile products of changed largely along with the process of decompose, the productions gradually increase, and the content changes much with the process of decompose. Denaturing gradient gel electrophoresis （DGGE） detected the dynamic change of bacterium compose, the bacterium changes much in different period, the result of Blast from 16S rDNA sequence was found that the closest relative in community belong to Clostridium sp., Brevibacillus sp., Rhizobium sp., Bacterium sp. four genera.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2009年第8期2458-2463,共6页
Environmental Science
基金
"十一五"国家科技支撑计划重大项目(2006BAD07A01
2006BAD10B05-2)
东北林业大学青年基金项目(07035)
教育部博士点新教师基金项目(20070019055)
关键词
常温菌群
木质纤维素分解
稻秆
挥发性产物
normal temperature microbial community
lignocellulose degradation
rice straw
volatile products
