摘要
为分析三磷酸-甘油醛脱氢酶(GAPDH)基因与小麦(Triticum aestivum)生理型雄性不育花药败育的关系,本研究以新型小麦化学杀雄剂SQ-1作为诱导剂,普通小麦西农1376为材料,构建了西农1376不育和可育等生理系;采用半定量逆转录聚合酶链式反应(RT-PCR)技术分析了不育和可育等生理系不同发育时期花药中GAPDH基因的表达模式。结果表明,该基因在正常小麦花粉发育的单核期、二核期和三核期,表达丰度比较一致,没有明显变化,但在化学杀雄剂SQ-1诱导的生理型雄性不育花药中,不同发育期表达量存在显著差异,单核期表达最弱,二核期最高。与同期正常花药相比,GAPDH基因在生理型雄性不育花药三个发育期均呈下调表达,其中在大量花粉粒败育的单核期,表达量下调最显著,其次为三核期和二核期,证明化学杀雄剂SQ-1对GAPDH基因的表达具有明显抑制作用。因此,化学杀雄剂SQ-1诱导小麦生理型雄性不育形成过程中,可能与GAPDH基因表达减少导致细胞中能量供给不足有一定关系。
The study was conducted in order to reveal the relationship between three phosphoric acid-glycerol dehydrogenase （GAPDH） gene related to energy metabolism pathway and physiological male sterility of Tritieum aestivum, the wheat variety Xinong1376 was explored to develop physiological male sterile line through spraying SQ-1, a new chemical agents （CHA）, on wheat plants at Feeke＇s8.5 stage, the plants sprayed with same volume water used as the control; RT-PCR technique was performed to analyze the expression patterns of the GAPDH gene in sterile and fertile anthers at different development stage. The results indicated that the transcripts abundance of GAPDH gene in anthers of the control was approximately same, but that in the anthers of treated plants with CHA SQ-1 was detected to be remarkbly different at different developmental stage; the transcripts of GAPDH in abundance in the treated anthers was lower than that of the control at the same developmental stage, especially the transcripts abundance of GAPDH of the treated anthers was very significantly lower than that of the control at the mononuclear stage, when a large of proportion of pollen have became aborted. Therefore, CHA SQ-1 can affect the course of glycolysis by inhibitting the expression of GAPDH gene of wheat anthers at different developmental stage of pollen, especially at the mononuclear stage, which results in energy shortage during the course of pollen development, ultimately leads to pollen abortion. The decreased transcripts ofGAPDH gene in wheat anthers were close interaction with pollen abortion.
出处
《分子植物育种》
CAS
CSCD
2009年第4期679-684,共6页
Molecular Plant Breeding
基金
陕西省13115科技创新工程重大科技专项(2007ZDKG-020)
国家杨凌农业生物技术育种中心专项基金(99-1A)
西北农林科技大学拔尖人才支持计划项目共同资助
