摘要
目的探讨纳米α-亚麻酸对病毒性心肌炎(VM)小鼠巨噬细胞移动抑制因子(MIF)表达的影响。方法80只雄性Balb/c小鼠随机分为对照组、模型组、低剂量干预组及高剂量干预组,每组20只。对照组小鼠腹腔接种病毒培养液,余3组经腹腔接种柯萨奇病毒(CVB3)诱发VM,低、高剂量干预组于病毒接种当天分别以60、180mg/kg纳米α-亚麻酸0.1mL灌胃,对照组和模型组均以9g/L盐水0.1mL灌胃,1次/d,连续7d,第15天处死全部存活小鼠,采用RT-PCR和免疫组织化学法检测其心肌MIFmRNA及蛋白表达,采用ELISA法测定其血清MIF水平。行HE染色检查心肌病理变化。结果对照组、模型组、低剂量干预组、高剂量干预组小鼠死亡率分别为0、45%、30%、20%,高剂量干预组死亡率显著低于模型组(P<0.05),低剂量干预组和模型组比较无显著性差异(P>0.05);模型组心肌MIF mRNA和蛋白表达水平、血清MIF水平均显著高于对照组(Pa<0.01),而低、高剂量干预组MIF mRNA和蛋白表达水平、血清MIF水平、心肌病理积分均较模型组显著降低(Pa<0.05)。结论纳米α-亚麻酸可通过抑制MIF表达减轻VM小鼠心肌损害,提高VM小鼠生存率。
Objective To investigate influence of nano-α-linolenic acid on expression of macrophage migration inhibitory factor （MIF） in murine model with viral myocarditis （VM）.Methods Eighty male Balb/c mice were randomly divided into 4 groups equally：control group,model group,low and high dose intervention group.Mice in control group were inoculated introperitoneally with Eagle＇s solution.Every mouse in other groups was treated with 0.1 mL Coxsackie B3 virus（CVB3） intraperitoneally.Then,mice in low and high dose intervention group were treated with 60 and 180 mg/kg nano-α-linolenic acid solution for 1 week,respectively.Mice in control group and model group were treated with 9 g/L saline for 1 week.All mice were killed on day 15.The expression levels of myocardial MIF mRNA and protein were detected by reverse transcriptase polymerase chain reaction（RT-PCR） and immunohistochemistry.Serum MIF concentration were exa-mined by enzymelinked immunosorbent assay（ELISA）.Myocardial histopathology was determined with hematoxylin and eosin stain.Results The mortality rate was 0,45%,30% and 20% in control group,model group,low and high dose intervention group,respectively.The mortality rate was significantly lower in high dose intervention group compared with model group （P〈0.05）,but there was no difference between low dose intervention group and model group.The expression of MIF mRNA and protein and serum MIF concentration were markedly higher in model group than those in control group（Pa〈0.01）.The expression of MIF mRNA and protein,serum MIF concentration,and myocardial histopathological scores were decreased significantly compared with model group in low and high dose intervention group（Pa〈0.05）.Conclusions Nano-α-linolenic acid can attenuate myocardial injure and increase survival rate through suppressing excessive expression of MIF.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2009年第13期976-978,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30271665)
