摘要
目的研究新疆维吾尔自治区维吾尔族（以下简称维族）阿尔茨海默病（AD）患者低密度脂蛋白受体相关蛋白（LRP）基因766C／T多态性及血脂水平。方法在流行病学调查基础上，采用美国国立神经病学和语言障碍与脑卒中研究所-阿尔茨海默病和相关障碍协会制定的标准，诊断为很可能AD的患者111例和正常对照者117名，应用聚合酶链反应-限制性片段长度多态性分析方法，检测两组LRP基因766C／T多态性；并用氧化酶法测定总胆固醇（TC）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL—C）的水平。结果（1）AD组LRP基因第766位点CC、CT、TT基因型频率（x^2=1．563）和等位基因频率（x^2=1．591）与对照组比较，差异均无统计学意义（P均〉0．05）。（2）AD组血清TC水平[（4．4±0．7）mmol／L]高于对照组[（4．2±0．6）mmol／L；t=2．234，P〈0．05]，TG水平[（1．81±0．33）mmol／L]亦高于对照组[（1．61±0．52）mmol／L；t=2．360，P〈0．05]。（3）AD组[（1．83±0．34）mmol／L]和对照组CC基因型者的血清TG水平[（1．67±0．54）mmol／L]均高于CT＋TT型者[分别为（1．66±0．24）mmoL／L和（1．41±0．39）mmol／L；t=2．275，2．161，P〈0．05]。结论LRP基因766C／T基因型频率和等位基因频率在AD组和对照组间的分布相似；AD患者存在脂质代谢紊乱，LRP基因766C／T多态性可能对血脂代谢有影响。
Objective To investigate lipoprotein-reeeptor related protein （LRP） gene 766C/T polymorphisms and the levels of serum lipids in Alzheimer disease （ AD ） in Xinjiang Uygurs. Methods The LRP gene 766C/T polymorphism was analyzed in 111 eases diagnosed according to the National Institute of Neurological and Cormnunieative Diseases and Stroke/Alzheimer Disease and Related Disorders Association（NINCDS/ADRDA） criteria and 117 normal controls by using polymerase chain reactionrestriction fragment length polymorphism on the basis of an epidemiologieal survey. The serum levels of total cholesterol, triglyeeride, low density lipopretein cholesterol and high density lipoprotein cholesterol were measured. Results （ 1 ） There were no statistical differences in genotype frequencies and allele frequencies between the AD group and control group （ P 〉 0.05 ）. （ 2 ） There were significant differences in the level of total cholesterol and triglyeeride between the AD group and control group （ P 〈 0.05 ）. （ 3 ） The serum levels of triglycefide with CC genotype were significantly higher than those with CT/TT genotype in the AD group and control group （ P 〈 0. 05 ）. Conclusions There may be no differences in distribution of LRP gene 766C/T genotype and allele frequency polymorphism between the AD group and control group, but AD patients may have the plasma lipoproteins metabolism disorder. LRP gene 766C/T polymorphisms maybe have effects on plasma lipoproteins metabolism.
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2009年第3期129-133,共5页
Chinese Journal of Psychiatry
基金
国家自然科学基金资助项目（30460140）
关键词
阿尔茨海默病
维吾尔族
受体
LDL
基因
多态性
限制性片段长度
脂蛋白类
胆固醇
Alzheimer disease
UYGUR NATIONALITY
Receptors, LDL
Genes
Polymorphism, restriction fragment length
Lipoproteins
Cholesterol
