摘要
目的探讨白细胞介素10(interleukin-10,IL-10)基因1082G/A位点单核苷酸多态性与汉族人群重度慢性牙周炎(chronic periodontitis,CP)发病风险的关系。方法收集汉族重度慢性牙周炎患者146例及牙周健康对照者138人的颊黏膜拭子,以Chelex-100法提取基因组DNA,采用聚合酶链反应-限制性片段长度多态性(poly-merase chain reaction restriction fragment length polymorphism,PCR-RFLP)分析法测定IL-10-1082G/A基因型,并分析组间基因型和等位基因频率的差异。结果CP组和对照组AA基因型分布分别率为91.8%和89.1%,P=0.45;A等位基因频率分别为95.9%和94.6%,P=0.46,差异无统计学意义。结论IL-10-1082G/A位点单核苷酸多态性与汉族人群重度慢性牙周炎发病风险无相关关系。
Objective To investigate the association between IL-10-1082 G/A single nucleotide polymorphism and severe CP in a Chinese population. Methods Buccal swab samples were taken from 146 severe CP patients and 138 control subjects. The DNA was extracted from the buccal swab samples using Chelex-100 resin method. The 1082 G/A site in the IL-10 promoter sequences was analyzed using polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP）. The digested fragments were electrophoresed on 3% agarose gel containing goldview and observed under ultraviolet light. Results The distribution of 1082 G/A site in the [L-IO promoter sequences genotypes and alleles between the two groups exhibted no significantly difference （ P = 0.45 and P = 0.46, respectively）. Conclusion The 1082 G/A single nucleotide polymorphism in the 1L-10 gene promoter do not appear to be associated with severe chronic periodontitis in Chinese population.
出处
《广东牙病防治》
2009年第8期354-357,共4页
Journal of Dental Prevention and Treatment
基金
"十一五"国家科技支撑项目(2007BAI18B02)
