摘要
本研究旨在分析RhD(-)非血缘献血者中Del的检测,采用血清学方法初筛RhD,用间接抗人球蛋白试验(IAT)确认RhD(-),应用热-乙醚吸收放散法检测Del。结果表明:在38526名健康无偿献血者中,常规血清学方法初筛和IAT试验确认RhD(-)者为106人,热-乙醚吸收放散试验确认Del型者28例,在RhD(-)中的比例为26.41%,Del中各表型的频率分别是Ccee22例,占78.57%,CCee4例,占14.29%,CcEe2例,占7.14%。结论:用热-乙醚吸收放散实验检测Del对合理运用Rh阴性血源具有重要意义,本地区人群的Del表型频率与中国不同区域人群及日本人群Del表型频率有差异。
The study was aimed to analyz Del phenotype of RhD （ - ） unrelated blood donors. RhD （ - ） was initially screened by routine serological test and confirmed by indirect antiglobulin test （IAT）. Del phenotype was detected by hot-ether absorption-elution test. The results indicated that 106 RhD（ - ）samples were confirmed out of 38526 donors, and 28 cases were Del detected by hot-ether absorption-elution test. The incidence of Del in RhD （ - ） samples was 26. 41%, The serological phenotypes of Del were Ccee（78.57% ）,CCee （14.29%）and CcEe （7.14%） respectively. In conclusion, the detection of Del by using hot-ether absorption-elution test is very important for reasonable application of RhD（ - ）blood. There is difference in Del phenotypes of populations in different regions of China and Japan.
出处
《中国实验血液学杂志》
CAS
CSCD
2009年第4期1071-1073,共3页
Journal of Experimental Hematology
