摘要
目的探讨乙型肝炎(简称乙肝)患者血清乙肝病毒(HBV)DNA载量与白细胞介素6(IL-6)、IL-8及肿瘤坏死因子-α(TNF-α)含量变化的相关性,揭示HBV感染者在HBV载量不同时的炎性反应活动状况。方法采用酶联免疫吸附试验(ELISA)检测71例HBV感染者及18例健康对照IL-6、IL-8及TNF-α含量。结果血清HBV DNA<1.0×103IU/mL组IL-6、IL-8及TNF-α含量与健康对照组比较,差异无统计学意义(P>0.05);血清HBV DNA>1.0×103IU/mL的其他3组IL-6、IL-8及TNF-α含量与健康对照组及HBV DNA<1.0×103IU/mL组比较,差异有统计学意义(P<0.01或P<0.05);血清HBV DNA有复制的3组间IL-6、IL-8及TNF-α含量差异无统计学意义(P>0.05)。结论HBV感染者血清IL-6、IL-8及TNF-α含量与HBV DNA载量呈正相关关系,其含量的动态变化可作为临床对HBV感染者炎性反应活动的观察指标。
Objective To explor the relationship between content changes of serum HBV-DNA loads and IL- 6,IL-8 and TNF alpha in the patients with Hepatitis B. Methods Using ELISA to test the content of IL-6, IL-8 and TNF alpha in 71 cases of hepatitis B virus infection and 18 normal controls. Results There were no statistical differences about Serum IL-8,IL-8 and TNF alpha between normal control and HBV DNA 〈1.0 × 10^3 groups （P〉 0.05）, there were statistical differences about Serum IL-6, IL-8 and TNF alpha between Serum HBV DNA 〉 1.0 × 10^3 and normal control ,and HBV DNA 〈1.0 × 10^3 groups（P〈0.01 or P〈0.05）. there were no statistical differences about Serum IL-6, IL-8 and TNF alpha between the three groups containing Serum HBV DNA copy（P〉 0.05）. Conclusion IL-6, IL-8 and TNF alpha content have positive relationship with HBV DNA loads in the patients with Hepatitis B. The dynamic changes of the cell factors content in hepatitis b virus can be used as a clinical observation of infected inflammation activities.
出处
《检验医学与临床》
CAS
2009年第16期1336-1337,共2页
Laboratory Medicine and Clinic
