摘要
本研究应用直接竞争ELISA法对河豚毒素（tetrodotoxin，TTX）进行快速检测。用过碘酸钠氧化法合成酶标记抗原（HRP-OVA-TFX），用直接ELISA方法对合成的酶标记抗原进行鉴定表明其与抗TTX单克隆抗体具有特异性反应。在比基础上建立了包被单抗的直接竞争ELISA方法。该方法对TTX的检测限可达到1.1μg／L，IC50为20.4μg／L，线性范围3.3-137μg／L，批内变异系数小于6.25％，批间变异系数小于7.34%。对鲫鱼的肌肉组织添加TTX标准品，回收率为65％-93.2％，变异系数为9.41％-12.77％。
A direct competitive enzyme-linked immunosobent assay （ELISA） method was developed for the rapid detection of the tetrodotoxin （TTX） in this paper. A horseradish peroxidase（HRP）-labeled conjugated antigen was synthesized by the sodium periodate reaction. The direct competitive ELISA was conducted by simultaneously incubating solided anfi-TTX monoclonal antibody with TTX and HRP-OVA-TTX. The detection limit for TTX was 1.1μg/L. The inter-and intra-assay coefficient of variation were 〈6.25% and 〈 7.34% in the range of 3.3-37μg/L, respectively. Recoveries from crucian muscle were in the range of 65%-93.2% and the coefficient of variation was in the range of 9.41%-12.77%.
出处
《现代食品科技》
EI
CAS
2009年第8期977-981,共5页
Modern Food Science and Technology
